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Assay Detail

CHEMBL4313574

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of porcine brain tubulin polymerization assessed as decrease in fluorescence intensity measured every 60 sec for 90 mins in presence of GTP by DAPI staining based fluorescence assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Sus scrofa
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Tubulin (CHEMBL2111354)
Type PROTEIN FAMILY
Organism Sus scrofa

Publication

Potent combretastatin A-4 analogs containing 1,2,4-triazole: Synthesis, antiproliferative, anti-tubulin activity, and docking study.
Mustafa M, Anwar S, Elgamal F, Ahmed ER, Aly OM.
Eur J Med Chem (2019) 183 : 111697 -111697
PubMed 31536891 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 6.14 6.28

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL67 COMBRETASTATIN A4 -1.0 1 6.28
CHEMBL4530425 1 6.16
CHEMBL4472670 1 6.12
CHEMBL4463939 1 5.99

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL67 COMBRETASTATIN A4 IC50 = 520.0 nM 6.28
CHEMBL4530425 IC50 = 700.0 nM 6.16
CHEMBL4472670 IC50 = 760.0 nM 6.12
CHEMBL4463939 IC50 = 1030.0 nM 5.99