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Assay Detail

CHEMBL4320985

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to His-tagged BLM (642 to 1296 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) by ITC assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

RecQ-like DNA helicase BLM (CHEMBL1293237)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Isaindigotone Derivatives as Novel Bloom's Syndrome Protein (BLM) Helicase Inhibitors That Disrupt the BLM/DNA Interactions and Regulate the Homologous Recombination Repair.
Yin QK, Wang CX, Wang YQ, Guo QL, Zhang ZL, Ou TM, Huang SL, Li D, Wang HG, Tan JH, Chen SB, Huang ZS.
J Med Chem (2019) 62 : 3147 -3162
PubMed 30827110 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 1 5.75 5.75

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4075790 1 5.75

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4075790 Kd = 1800.0 nM 5.75