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Assay Detail

CHEMBL4323740

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to recombinant full length human CYP51 expressed in Escherichia coli incubated for 30 sec using [3H] labelled substrate by HPLC analysis
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lanosterol 14-alpha demethylase (CHEMBL3849)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Validation of Human Sterol 14α-Demethylase (CYP51) Druggability: Structure-Guided Design, Synthesis, and Evaluation of Stoichiometric, Functionally Irreversible Inhibitors.
Friggeri L, Hargrove TY, Wawrzak Z, Guengerich FP, Lepesheva GI.
J Med Chem (2019) 62 : 10391 -10401
PubMed 31663733 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 12 6.45 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4461447 1 7.70
CHEMBL4277680 1 7.22
CHEMBL3629567 1 7.05
CHEMBL4522116 1 7.05
CHEMBL4439225 1 6.89
CHEMBL4435160 1 6.82
CHEMBL4444489 1 6.33
CHEMBL4593688 1 5.94
CHEMBL4458275 1 5.78
CHEMBL4472919 1 5.63
CHEMBL4466288 1 5.60
CHEMBL4452294 1 5.41

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4461447 Kd = 20.0 nM 7.70
CHEMBL4277680 Kd = 60.0 nM 7.22
CHEMBL3629567 Kd = 90.0 nM 7.05
CHEMBL4522116 Kd = 90.0 nM 7.05
CHEMBL4439225 Kd = 130.0 nM 6.89
CHEMBL4435160 Kd = 150.0 nM 6.82
CHEMBL4444489 Kd = 470.0 nM 6.33
CHEMBL4593688 Kd = 1150.0 nM 5.94
CHEMBL4458275 Kd = 1650.0 nM 5.78
CHEMBL4472919 Kd = 2320.0 nM 5.63
CHEMBL4466288 Kd = 2500.0 nM 5.60
CHEMBL4452294 Kd = 3900.0 nM 5.41