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Assay Detail

CHEMBL4324122

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of phosphorylated EGFR del19/T790M/C797S triple mutant (unknown origin) expressed in mouse BaF3 cells incubated for 4 hrs by Alphascreen assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type BaF3
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Start Selective and Rigidify: The Discovery Path toward a Next Generation of EGFR Tyrosine Kinase Inhibitors.
Engelhardt H, Böse D, Petronczki M, Scharn D, Bader G, Baum A, Bergner A, Chong E, Döbel S, Egger G, Engelhardt C, Ettmayer P, Fuchs JE, Gerstberger T, Gonnella N, Grimm A, Grondal E, Haddad N, Hopfgartner B, Kousek R, Krawiec M, Kriz M, Lamarre L, Leung J, Mayer M, Patel ND, Simov BP, Reeves JT, Schnitzer R, Schrenk A, Sharps B, Solca F, Stadtmüller H, Tan Z, Wunberg T, Zoephel A, McConnell DB.
J Med Chem (2019) 62 : 10272 -10293
PubMed 31689114 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 8.02 9.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4537790 1 9.22
CHEMBL4562138 1 9.10
CHEMBL4550104 1 9.00
CHEMBL4532034 1 9.00
CHEMBL4566193 1 8.92
CHEMBL4519157 1 8.52
CHEMBL4434788 1 7.66
CHEMBL4449589 1 7.46
CHEMBL4525935 1 7.37
CHEMBL4591327 1 6.10
CHEMBL4444231 1 5.85
CHEMBL3353410 OSIMERTINIB 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4537790 IC50 = 0.6 nM 9.22
CHEMBL4562138 IC50 = 0.8 nM 9.10
CHEMBL4550104 IC50 = 1.0 nM 9.00
CHEMBL4532034 IC50 = 1.0 nM 9.00
CHEMBL4566193 IC50 = 1.2 nM 8.92
CHEMBL4519157 IC50 = 3.0 nM 8.52
CHEMBL4434788 IC50 = 22.0 nM 7.66
CHEMBL4449589 IC50 = 35.0 nM 7.46
CHEMBL4525935 IC50 = 43.0 nM 7.37
CHEMBL4591327 IC50 = 790.0 nM 6.10
CHEMBL4444231 IC50 = 1400.0 nM 5.85
CHEMBL3353410 OSIMERTINIB IC50 = 3100.0 nM -