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Assay Detail

CHEMBL4324285

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of MEK U911-activated ERK2 (unknown origin) using ERKtide as substrate preincubated for 20 mins followed by substrate addition in presence of 1 mM ATP after 10 mins by rapidfire mass spectrometry analysis
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mitogen-activated protein kinase 1 (CHEMBL4040)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a Potent and Selective Oral Inhibitor of ERK1/2 (AZD0364) That Is Efficacious in Both Monotherapy and Combination Therapy in Models of Nonsmall Cell Lung Cancer (NSCLC).
Ward RA, Anderton MJ, Bethel P, Breed J, Cook C, Davies EJ, Dobson A, Dong Z, Fairley G, Farrington P, Feron L, Flemington V, Gibbons FD, Graham MA, Greenwood R, Hanson L, Hopcroft P, Howells R, Hudson J, James M, Jones CD, Jones CR, Li Y, Lamont S, Lewis R, Lindsay N, McCabe J, McGuire T, Rawlins P, Roberts K, Sandin L, Simpson I, Swallow S, Tang J, Tomkinson G, Tonge M, Wang Z, Zhai B.
J Med Chem (2019) 62 : 11004 -11018
PubMed 31710489 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 9.33 9.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4470113 1 9.89
CHEMBL4440109 1 9.49
CHEMBL4452853 1 9.47
CHEMBL4587118 1 9.46
CHEMBL4071576 1 9.30
CHEMBL4551714 1 9.22
CHEMBL4482864 TIZATERKIB 1.0 1 9.18
CHEMBL4458562 1 8.59

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4470113 IC50 = 0.13 nM 9.89
CHEMBL4440109 IC50 = 0.32 nM 9.49
CHEMBL4452853 IC50 = 0.34 nM 9.47
CHEMBL4587118 IC50 = 0.35 nM 9.46
CHEMBL4071576 IC50 = 0.5 nM 9.30
CHEMBL4551714 IC50 = 0.6 nM 9.22
CHEMBL4482864 TIZATERKIB IC50 = 0.66 nM 9.18
CHEMBL4458562 IC50 = 2.6 nM 8.59