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Assay Detail

CHEMBL4324286

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Binding
Inhibition of ERK1/2 in human A375 cells assessed as inhibition of ERK phosphorylation after 2 hrs
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Mitogen-activated protein kinase; ERK1/ERK2 (CHEMBL1907606)
Type PROTEIN FAMILY
Organism Homo sapiens

Publication

Discovery of a Potent and Selective Oral Inhibitor of ERK1/2 (AZD0364) That Is Efficacious in Both Monotherapy and Combination Therapy in Models of Nonsmall Cell Lung Cancer (NSCLC).
Ward RA, Anderton MJ, Bethel P, Breed J, Cook C, Davies EJ, Dobson A, Dong Z, Fairley G, Farrington P, Feron L, Flemington V, Gibbons FD, Graham MA, Greenwood R, Hanson L, Hopcroft P, Howells R, Hudson J, James M, Jones CD, Jones CR, Li Y, Lamont S, Lewis R, Lindsay N, McCabe J, McGuire T, Rawlins P, Roberts K, Sandin L, Simpson I, Swallow S, Tang J, Tomkinson G, Tonge M, Wang Z, Zhai B.
J Med Chem (2019) 62 : 11004 -11018
PubMed 31710489 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 7.85 8.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4482864 TIZATERKIB 1.0 1 8.77
CHEMBL3590107 1 8.60
CHEMBL4440109 1 8.47
CHEMBL4452853 1 8.33
CHEMBL4458562 1 8.04
CHEMBL4071576 1 7.92
CHEMBL4551714 1 7.52
CHEMBL3544964 RAVOXERTINIB 2.0 1 7.51
CHEMBL3590106 ULIXERTINIB 2.0 1 5.51
CHEMBL4470113 1 -
CHEMBL4587118 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4482864 TIZATERKIB IC50 = 1.7 nM 8.77
CHEMBL3590107 IC50 = 2.5 nM 8.60
CHEMBL4440109 IC50 = 3.4 nM 8.47
CHEMBL4452853 IC50 = 4.7 nM 8.33
CHEMBL4458562 IC50 = 9.1 nM 8.04
CHEMBL4071576 IC50 = 12.0 nM 7.92
CHEMBL4551714 IC50 = 30.0 nM 7.52
CHEMBL3544964 RAVOXERTINIB IC50 = 31.0 nM 7.51
CHEMBL3590106 ULIXERTINIB IC50 = 3100.0 nM 5.51
CHEMBL4470113 IC50 > 14000.0 nM -
CHEMBL4587118 IC50 - -