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Assay Detail

CHEMBL4325227

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at recombinant human Gi/Go-coupled OTR expressed in CHOK1 cells assessed as induction of beta-arrestin recruitment measured after 90 mins in presence of 10% FBS by pathhunter assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Oxytocin receptor (CHEMBL2049)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Engineering a Potent, Long-Acting, and Periphery-Restricted Oxytocin Receptor Agonist with Anorexigenic and Body Weight Reducing Effects.
Pflimlin E, Zhou Z, Amso Z, Fu Q, Lee C, Muppiddi A, Joseph SB, Nguyen-Tran V, Shen W.
J Med Chem (2020) 63 : 382 -390
PubMed 31850759 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 14 7.50 8.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL395429 OXYTOCIN 4.0 1 8.64
CHEMBL3301668 CARBETOCIN 3.0 1 8.52
CHEMBL4460622 1 8.32
CHEMBL4463103 1 8.28
CHEMBL4550811 1 7.84
CHEMBL4546106 1 7.72
CHEMBL4442222 1 7.25
CHEMBL4546597 1 6.76
CHEMBL4537162 1 6.71
CHEMBL4456573 1 6.33
CHEMBL4560445 1 6.08
CHEMBL4541779 1 -
CHEMBL4589487 1 -
CHEMBL4532581 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL395429 OXYTOCIN EC50 = 2.29 nM 8.64
CHEMBL3301668 CARBETOCIN EC50 = 2.99 nM 8.52
CHEMBL4460622 EC50 = 4.8 nM 8.32
CHEMBL4463103 EC50 = 5.28 nM 8.28
CHEMBL4550811 EC50 = 14.42 nM 7.84
CHEMBL4546106 EC50 = 18.93 nM 7.72
CHEMBL4442222 EC50 = 56.94 nM 7.25
CHEMBL4546597 EC50 = 173.4 nM 6.76
CHEMBL4537162 EC50 = 192.6 nM 6.71
CHEMBL4456573 EC50 = 463.8 nM 6.33
CHEMBL4560445 EC50 = 831.2 nM 6.08
CHEMBL4541779 EC50 > 1000.0 nM -
CHEMBL4589487 EC50 > 1000.0 nM -
CHEMBL4532581 EC50 > 1000.0 nM -