Assay Detail
Binding
CHEMBL4326150
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of [3H]prostaglandin E2 from human EP2 receptor expressed in HEK293 cell membranes after 60 mins by liquid scintillation counting
6
Total Activities
6
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Prostaglandin E2 receptor EP2 subtype (CHEMBL1881) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Identification of a Selective, Non-Prostanoid EP2 Receptor Agonist for the Treatment of Glaucoma: Omidenepag and its Prodrug Omidenepag Isopropyl.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 3 | 7.33 | 7.82 |
| IC50 | 3 | 7.26 | 8.40 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL548 | DINOPROSTONE | 4.0 | 1 | 8.40 |
| CHEMBL3707245 | OMIDENEPAG | 2.0 | 1 | 8.00 |
| CHEMBL4443289 | — | — | 1 | 7.82 |
| CHEMBL2105672 | EVATANEPAG SODIUM | -1.0 | 1 | 7.80 |
| CHEMBL4448995 | — | — | 1 | 6.36 |
| CHEMBL4297666 | OMIDENEPAG ISOPROPYL | 4.0 | 1 | 5.38 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL548 | DINOPROSTONE | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3707245 | OMIDENEPAG | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL4443289 | — | Ki | = | 15.0 | nM | 7.82 |
| CHEMBL2105672 | EVATANEPAG SODIUM | Ki | = | 16.0 | nM | 7.80 |
| CHEMBL4448995 | — | Ki | = | 433.0 | nM | 6.36 |
| CHEMBL4297666 | OMIDENEPAG ISOPROPYL | IC50 | = | 4180.0 | nM | 5.38 |