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Assay Detail

CHEMBL4327418

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ZIKV 2'-O-MTase assessed as reduction in [3H]AdoMet transfer on to capped GpppAC4 RNA after 30 mins by microbeta liquid scintillation counting method
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Zika virus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Genome polyprotein (CHEMBL4523307)
Type SINGLE PROTEIN
Organism Zika virus

Publication

Optimization of a fragment linking hit toward Dengue and Zika virus NS5 methyltransferases inhibitors.
Hernandez J, Hoffer L, Coutard B, Querat G, Roche P, Morelli X, Decroly E, Barral K.
Eur J Med Chem (2019) 161 : 323 -333
PubMed 30368131 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 4.34 4.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4556728 1 4.72
CHEMBL3899268 1 4.62
CHEMBL4446745 1 4.55
CHEMBL3971531 1 4.51
CHEMBL3959384 1 4.48
CHEMBL4465538 1 4.21
CHEMBL3941938 1 4.17
CHEMBL4454666 1 4.08
CHEMBL3976785 1 4.06
CHEMBL4522746 1 4.02
CHEMBL4475329 1 -
CHEMBL4551201 1 -
CHEMBL4578774 1 -
CHEMBL4538799 1 -
CHEMBL4449695 1 -
CHEMBL4436568 1 -
CHEMBL4437452 1 -
CHEMBL4522025 1 -
CHEMBL4436970 1 -
CHEMBL4513879 1 -
CHEMBL3890313 1 -
CHEMBL4547767 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4556728 IC50 = 19000.0 nM 4.72
CHEMBL3899268 IC50 = 24000.0 nM 4.62
CHEMBL4446745 IC50 = 28000.0 nM 4.55
CHEMBL3971531 IC50 = 31000.0 nM 4.51
CHEMBL3959384 IC50 = 33000.0 nM 4.48
CHEMBL4465538 IC50 = 61000.0 nM 4.21
CHEMBL3941938 IC50 = 67000.0 nM 4.17
CHEMBL4454666 IC50 = 83000.0 nM 4.08
CHEMBL3976785 IC50 = 87000.0 nM 4.06
CHEMBL4522746 IC50 = 95000.0 nM 4.02
CHEMBL4475329 IC50 = 136000.0 nM -
CHEMBL4551201 IC50 - -
CHEMBL4578774 IC50 = 400000.0 nM -
CHEMBL4538799 IC50 = 400000.0 nM -
CHEMBL4449695 IC50 - -
CHEMBL4436568 IC50 = 135000.0 nM -
CHEMBL4437452 IC50 = 122000.0 nM -
CHEMBL4522025 IC50 = 185000.0 nM -
CHEMBL4436970 IC50 = 400000.0 nM -
CHEMBL4513879 IC50 = 111000.0 nM -
CHEMBL3890313 IC50 = 221000.0 nM -
CHEMBL4547767 IC50 = 132000.0 nM -