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Assay Detail

CHEMBL4329316

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human erythrocytes AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured at 1 min time interval for 10 mins by Ellman's method
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acetylcholinesterase (CHEMBL220)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel multitarget-directed ligands targeting acetylcholinesterase and σ<sub>1</sub> receptors as lead compounds for treatment of Alzheimer's disease: Synthesis, evaluation, and structural characterization of their complexes with acetylcholinesterase.
Lalut J, Santoni G, Karila D, Lecoutey C, Davis A, Nachon F, Silman I, Sussman J, Weik M, Maurice T, Dallemagne P, Rochais C.
Eur J Med Chem (2019) 162 : 234 -248
PubMed 30447434 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 7.11 8.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL502 DONEPEZIL 4.0 1 8.22
CHEMBL4585990 1 7.88
CHEMBL4526050 1 7.69
CHEMBL4444843 1 7.54
CHEMBL4454706 1 7.40
CHEMBL4529710 1 7.29
CHEMBL4559904 1 7.07
CHEMBL4537101 1 7.04
CHEMBL4562793 1 7.03
CHEMBL4578844 1 6.88
CHEMBL4541090 1 6.58
CHEMBL4540355 1 6.56
CHEMBL4583738 1 6.25
CHEMBL4567394 1 6.14
CHEMBL54 HALOPERIDOL 4.0 1 -
CHEMBL85251 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL502 DONEPEZIL IC50 = 6.0 nM 8.22
CHEMBL4585990 IC50 = 13.3 nM 7.88
CHEMBL4526050 IC50 = 20.4 nM 7.69
CHEMBL4444843 IC50 = 28.8 nM 7.54
CHEMBL4454706 IC50 = 40.0 nM 7.40
CHEMBL4529710 IC50 = 51.0 nM 7.29
CHEMBL4559904 IC50 = 84.4 nM 7.07
CHEMBL4537101 IC50 = 91.4 nM 7.04
CHEMBL4562793 IC50 = 94.0 nM 7.03
CHEMBL4578844 IC50 = 133.0 nM 6.88
CHEMBL4541090 IC50 = 260.6 nM 6.58
CHEMBL4540355 IC50 = 275.0 nM 6.56
CHEMBL4583738 IC50 = 559.0 nM 6.25
CHEMBL4567394 IC50 = 716.0 nM 6.14
CHEMBL54 HALOPERIDOL IC50 - -
CHEMBL85251 IC50 - -