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Assay Detail

CHEMBL4330040

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human ABHD16A expressed in HEK293 cells preincubated for 30 mins followed by 1-LG substrate addition and measured after 90 to 120 mins by fluorometric assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Phosphatidylserine lipase ABHD16A (CHEMBL6168)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 12-Thiazole Abietanes as Selective Inhibitors of the Human Metabolic Serine Hydrolase hABHD16A.
Ahonen TJ, Savinainen JR, Yli-Kauhaluoma J, Kalso E, Laitinen JT, Moreira VM.
ACS Med Chem Lett (2018) 9 : 1269 -1273
PubMed 30613338 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 5.93 7.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1673415 PALMOSTATIN B 1 7.00
CHEMBL4585683 1 5.47
CHEMBL4591378 1 5.31

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1673415 PALMOSTATIN B IC50 = 99.0 nM 7.00
CHEMBL4585683 IC50 = 3400.0 nM 5.47
CHEMBL4591378 IC50 = 4900.0 nM 5.31