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Assay Detail

CHEMBL4331684

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of F-NEO from Escherichia coli rRNA-A site after 10 to 20 mins by fluorescence assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 3 — Cell-line / Tissue
Curated By Autocuration

Publication

Synthesis, antimicrobial activity, attenuation of aminoglycoside resistance in MRSA, and ribosomal A-site binding of pyrene-neomycin conjugates.
Story S, Skriba MJ, Maiti K, Nihar Ranjan, Degtyareva NN, Green KD, Khodaverdian V, Oyelere AK, Garneau-Tsodikova S, Arya DP.
Eur J Med Chem (2019) 163 : 381 -393
PubMed 30530174 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 6.75 7.06

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL184618 FRAMYCETIN 3.0 1 7.06
CHEMBL4442608 1 7.02
CHEMBL4536151 1 6.83
CHEMBL445770 1 6.77
CHEMBL4457923 1 6.72
CHEMBL4459175 1 6.59
CHEMBL4438817 1 6.52
CHEMBL4527321 1 6.51

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL184618 FRAMYCETIN IC50 = 87.0 nM 7.06
CHEMBL4442608 IC50 = 95.0 nM 7.02
CHEMBL4536151 IC50 = 148.0 nM 6.83
CHEMBL445770 IC50 = 170.0 nM 6.77
CHEMBL4457923 IC50 = 191.0 nM 6.72
CHEMBL4459175 IC50 = 254.0 nM 6.59
CHEMBL4438817 IC50 = 303.0 nM 6.52
CHEMBL4527321 IC50 = 310.0 nM 6.51