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Assay Detail

CHEMBL4332981

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of JAK3 in human CTLL2 cells assessed as reduction in IL15-induced STAT5 phosphorylation preincubated for 2 hrs followed by IL15 stimulation measured after 30 mins by Western blot analysis
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase JAK3 (CHEMBL2148)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of an Orally Available Janus Kinase 3 Selective Covalent Inhibitor.
Shi L, Zhong Z, Li X, Zhou Y, Pan Z.
J Med Chem (2019) 62 : 1054 -1066
PubMed 30615446 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 2 6.42 6.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4450627 1 6.85
CHEMBL1650951 1 5.98

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4450627 EC50 = 141.0 nM 6.85
CHEMBL1650951 EC50 = 1042.0 nM 5.98