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Assay Detail

CHEMBL4340238

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Pseudomonas aeruginosa AmpC expressed in Escherichia coli BL21(DE3) using cephalothin as reporter substrate incubated for 300 secs by spectrophotometric assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Pseudomonas aeruginosa
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

X-ray Crystallography Deciphers the Activity of Broad-Spectrum Boronic Acid β-Lactamase Inhibitors.
Cendron L, Quotadamo A, Maso L, Bellio P, Montanari M, Celenza G, Venturelli A, Costi MP, Tondi D.
ACS Med Chem Lett (2019) 10 : 650 -655
PubMed 30996812 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 6 6.66 7.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL235073 1 7.40
CHEMBL4466682 1 7.30
CHEMBL4541975 1 7.16
CHEMBL4568968 1 6.38
CHEMBL4458371 1 5.89
CHEMBL4535625 1 5.85

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL235073 Ki = 40.0 nM 7.40
CHEMBL4466682 Ki = 50.0 nM 7.30
CHEMBL4541975 Ki = 70.0 nM 7.16
CHEMBL4568968 Ki = 420.0 nM 6.38
CHEMBL4458371 Ki = 1300.0 nM 5.89
CHEMBL4535625 Ki = 1400.0 nM 5.85