Assay Detail
Binding
CHEMBL4345287
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of 6N-FAM from human LRH1 LBD (299 to 541 residues) expressed in Escherichia coli BL21 pLysS by competitive binding based fluorescence polarization assay
9
Total Activities
8
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Nuclear receptor subfamily 5 group A member 2 (CHEMBL3544) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Development of a Versatile and Sensitive Direct Ligand Binding Assay for Human NR5A Nuclear Receptors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 8 | 6.70 | 8.68 |
| Inhibition | 1 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4551205 | — | — | 2 | 8.68 |
| CHEMBL4467442 | — | — | 1 | 7.19 |
| CHEMBL4288877 | — | — | 1 | 7.09 |
| CHEMBL1765959 | — | — | 1 | 6.43 |
| CHEMBL4452196 | — | — | 1 | 5.62 |
| CHEMBL4448429 | — | — | 1 | 5.16 |
| CHEMBL259067 | — | — | 1 | - |
| CHEMBL1325149 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4551205 | — | Ki | = | 2.1 | nM | 8.68 |
| CHEMBL4467442 | — | Ki | = | 64.3 | nM | 7.19 |
| CHEMBL4288877 | — | Ki | = | 81.4 | nM | 7.09 |
| CHEMBL1765959 | — | Ki | = | 374.0 | nM | 6.43 |
| CHEMBL4452196 | — | Ki | = | 2400.0 | nM | 5.62 |
| CHEMBL4448429 | — | Ki | = | 7000.0 | nM | 5.16 |
| CHEMBL4551205 | — | Inhibition | - | % | - | |
| CHEMBL259067 | — | Ki | - | — | - | |
| CHEMBL1325149 | — | Ki | - | — | - |