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Assay Detail

CHEMBL4351620

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Binding
Inhibition of biotinylated peptide binding to human GST-tagged PCAF bromodomain expressed in Escherichia coli Rosetta (DE3) measured after 15 hrs by HTRF assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone acetyltransferase KAT2B (CHEMBL5500)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Pyrrolo[3,2- d]pyrimidin-4-one Derivatives as a New Class of Potent and Cell-Active Inhibitors of P300/CBP-Associated Factor Bromodomain.
Huang L, Li H, Li L, Niu L, Seupel R, Wu C, Cheng W, Chen C, Ding B, Brennan PE, Yang S.
J Med Chem (2019) 62 : 4526 -4542
PubMed 30998845 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 7.55 8.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4574669 1 8.15
CHEMBL4543497 1 8.00
CHEMBL4449143 1 7.85
CHEMBL4439202 1 7.77
CHEMBL4545594 1 7.66
CHEMBL4453637 1 7.60
CHEMBL4570924 1 7.52
CHEMBL4527339 1 7.52
CHEMBL4454700 1 7.50
CHEMBL4454766 1 7.46
CHEMBL4570969 1 7.44
CHEMBL4471748 1 7.39
CHEMBL4457684 1 7.33
CHEMBL4565832 1 7.14
CHEMBL4541223 1 6.90

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4574669 IC50 = 7.0 nM 8.15
CHEMBL4543497 IC50 = 10.0 nM 8.00
CHEMBL4449143 IC50 = 14.0 nM 7.85
CHEMBL4439202 IC50 = 17.0 nM 7.77
CHEMBL4545594 IC50 = 22.0 nM 7.66
CHEMBL4453637 IC50 = 25.0 nM 7.60
CHEMBL4570924 IC50 = 30.0 nM 7.52
CHEMBL4527339 IC50 = 30.0 nM 7.52
CHEMBL4454700 IC50 = 32.0 nM 7.50
CHEMBL4454766 IC50 = 35.0 nM 7.46
CHEMBL4570969 IC50 = 36.0 nM 7.44
CHEMBL4471748 IC50 = 41.0 nM 7.39
CHEMBL4457684 IC50 = 47.0 nM 7.33
CHEMBL4565832 IC50 = 73.0 nM 7.14
CHEMBL4541223 IC50 = 125.0 nM 6.90