Assay Detail
Binding
CHEMBL4351666
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of Halo-tagged histone H3.3 binding to N-terminal Nano luciferase-fused full-length PCAF (unknown origin) expressed in HEK293T cells co-expressing C-terminal Halo-tagged histone H3.3 measured after 18 hrs by 618 fluorescent ligand-based BRET assay
3
Total Activities
2
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Discovery of Pyrrolo[3,2- d]pyrimidin-4-one Derivatives as a New Class of Potent and Cell-Active Inhibitors of P300/CBP-Associated Factor Bromodomain.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Inhibition | 2 | - | - |
| IC50 | 1 | 6.93 | 6.93 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4574669 | — | — | 2 | 6.93 |
| CHEMBL4581184 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4574669 | — | IC50 | = | 118.0 | nM | 6.93 |
| CHEMBL4581184 | — | Inhibition | - | % | - | |
| CHEMBL4574669 | — | Inhibition | - | % | - |