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Assay Detail

CHEMBL4352637

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of N-terminal His6-tagged recombinant full length human p110beta/untagged recombinant full length human p85alpha expressed in baculovirus infected Sf21 insect cells using PIP2 as substrate measured after 80 mins by transcreener fluorescence polarization assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Cell Type Sf21
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

PI3K p110 beta/p85 alpha (CHEMBL3038510)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Discovery, Optimization, and Evaluation of Potent and Highly Selective PI3Kγ-PI3Kδ Dual Inhibitors.
Jia H, Dai G, Su W, Xiao K, Weng J, Zhang Z, Wang Q, Yuan T, Shi F, Zhang Z, Chen W, Sai Y, Wang J, Li X, Cai Y, Yu J, Ren P, Venable J, Rao T, Edwards JP, Bembenek SD.
J Med Chem (2019) 62 : 4936 -4948
PubMed 31033293 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 6.88 8.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3039502 DUVELISIB 4.0 1 8.10
CHEMBL4471912 1 7.60
CHEMBL4514752 1 7.47
CHEMBL4446047 1 7.29
CHEMBL4438693 1 7.04
CHEMBL4463472 1 6.78
CHEMBL4520300 1 6.77
CHEMBL4522342 1 6.72
CHEMBL2216870 IDELALISIB 4.0 1 6.53
CHEMBL4549473 1 6.53
CHEMBL4548876 1 6.38
CHEMBL4513442 1 6.23
CHEMBL4445566 1 6.03
CHEMBL4469444 1 -
CHEMBL4459544 1 -
CHEMBL4541260 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3039502 DUVELISIB IC50 = 8.0 nM 8.10
CHEMBL4471912 IC50 = 25.0 nM 7.60
CHEMBL4514752 IC50 = 34.0 nM 7.47
CHEMBL4446047 IC50 = 51.0 nM 7.29
CHEMBL4438693 IC50 = 91.0 nM 7.04
CHEMBL4463472 IC50 = 165.0 nM 6.78
CHEMBL4520300 IC50 = 170.0 nM 6.77
CHEMBL4522342 IC50 = 191.0 nM 6.72
CHEMBL2216870 IDELALISIB IC50 = 293.0 nM 6.53
CHEMBL4549473 IC50 = 298.0 nM 6.53
CHEMBL4548876 IC50 = 420.0 nM 6.38
CHEMBL4513442 IC50 = 590.0 nM 6.23
CHEMBL4445566 IC50 = 945.0 nM 6.03
CHEMBL4469444 IC50 - -
CHEMBL4459544 IC50 - -
CHEMBL4541260 IC50 > 1000.0 nM -