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Assay Detail

CHEMBL4355542

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal GST-tagged recombinant human full-length ZAK expressed in baculovirus infected Sf9 insect cells using MBP as substrate after 30 mins by ADP-Glo assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mitogen-activated protein kinase kinase kinase 20 (CHEMBL3886)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, Synthesis, and Structure-Activity Relationships of 1,2,3-Triazole Benzenesulfonamides as New Selective Leucine-Zipper and Sterile-α Motif Kinase (ZAK) Inhibitors.
Yang J, Shibu MA, Kong L, Luo J, BadrealamKhan F, Huang Y, Tu ZC, Yun CH, Huang CY, Ding K, Lu X.
J Med Chem (2020) 63 : 2114 -2130
PubMed 31244114 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 7.87 8.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4524151 1 8.72
CHEMBL4542865 1 8.68
CHEMBL4526537 1 8.64
CHEMBL4574656 1 8.52
CHEMBL4579609 1 8.48
CHEMBL4446003 1 8.42
CHEMBL4450810 1 8.40
CHEMBL4468296 1 8.38
CHEMBL4454493 1 8.36
CHEMBL4448690 1 8.35
CHEMBL4457930 1 8.24
CHEMBL4449013 1 8.16
CHEMBL4559022 1 8.05
CHEMBL4096439 1 7.83
CHEMBL4476053 1 7.61
CHEMBL4473008 1 7.54
CHEMBL4457468 1 7.33
CHEMBL4532800 1 7.28
CHEMBL4523007 1 7.26
CHEMBL4452568 1 7.20
CHEMBL4444385 1 7.07
CHEMBL4582776 1 6.38
CHEMBL4459658 1 6.01

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4524151 IC50 = 1.9 nM 8.72
CHEMBL4542865 IC50 = 2.1 nM 8.68
CHEMBL4526537 IC50 = 2.3 nM 8.64
CHEMBL4574656 IC50 = 3.0 nM 8.52
CHEMBL4579609 IC50 = 3.3 nM 8.48
CHEMBL4446003 IC50 = 3.8 nM 8.42
CHEMBL4450810 IC50 = 4.0 nM 8.40
CHEMBL4468296 IC50 = 4.2 nM 8.38
CHEMBL4454493 IC50 = 4.4 nM 8.36
CHEMBL4448690 IC50 = 4.5 nM 8.35
CHEMBL4457930 IC50 = 5.7 nM 8.24
CHEMBL4449013 IC50 = 6.9 nM 8.16
CHEMBL4559022 IC50 = 8.9 nM 8.05
CHEMBL4096439 IC50 = 14.7 nM 7.83
CHEMBL4476053 IC50 = 24.7 nM 7.61
CHEMBL4473008 IC50 = 29.0 nM 7.54
CHEMBL4457468 IC50 = 46.2 nM 7.33
CHEMBL4532800 IC50 = 53.0 nM 7.28
CHEMBL4523007 IC50 = 54.3 nM 7.26
CHEMBL4452568 IC50 = 63.3 nM 7.20
CHEMBL4444385 IC50 = 85.7 nM 7.07
CHEMBL4582776 IC50 = 413.3 nM 6.38
CHEMBL4459658 IC50 = 983.0 nM 6.01