Assay Detail
Binding
CHEMBL4356007
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of N-terminal His6-tagged Zika virus NS2B (45 to 96 residues) - NS3 (1 to 177 residues) protease domain expressed in Escherichia coli BL21-Gold (DE3) cells or Escherichia coli BL21 Rosetta (DE3) preincubated for 30 mins followed by Bz-nKRR-AM substrate addition measured over 10 mins at 60 secs interval by fluorescence method
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Zika virus |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Drugs for the Treatment of Zika Virus Infection.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 6.41 | 7.00 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4435769 | — | — | 1 | 7.00 |
| CHEMBL569561 | — | — | 1 | 5.82 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4435769 | — | IC50 | = | 100.0 | nM | 7.00 |
| CHEMBL569561 | — | IC50 | = | 1500.0 | nM | 5.82 |