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Assay Detail

CHEMBL4356125

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human TDO expressed in Escherichia coli BL21 (DE3) assessed as reduction in conversion of L-tryptophan to N-formyl kynurenine using L-tryptophan as substrate after 30 mins by methylene blue assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tryptophan 2,3-dioxygenase (CHEMBL2140)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

N-Benzyl/Aryl Substituted Tryptanthrin as Dual Inhibitors of Indoleamine 2,3-Dioxygenase and Tryptophan 2,3-Dioxygenase.
Yang D, Zhang S, Fang X, Guo L, Hu N, Guo Z, Li X, Yang S, He JC, Kuang C, Yang Q.
J Med Chem (2019) 62 : 9161 -9174
PubMed 31580660 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 5.59 6.39

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4452150 1 6.39
CHEMBL4577825 1 6.35
CHEMBL4437046 1 6.12
CHEMBL4436480 1 6.06
CHEMBL4588805 1 6.06
CHEMBL4545004 1 5.62
CHEMBL4536439 1 5.59
CHEMBL4471831 1 5.50
CHEMBL4468741 1 5.42
CHEMBL4584081 1 5.19
CHEMBL4471895 1 5.17
CHEMBL4578338 1 5.00
CHEMBL3545369 EPACADOSTAT 3.0 1 4.19
CHEMBL504816 (L)-1-METHYLTRYPTOPHAN 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4452150 IC50 = 410.0 nM 6.39
CHEMBL4577825 IC50 = 450.0 nM 6.35
CHEMBL4437046 IC50 = 760.0 nM 6.12
CHEMBL4436480 IC50 = 870.0 nM 6.06
CHEMBL4588805 IC50 = 870.0 nM 6.06
CHEMBL4545004 IC50 = 2410.0 nM 5.62
CHEMBL4536439 IC50 = 2590.0 nM 5.59
CHEMBL4471831 IC50 = 3150.0 nM 5.50
CHEMBL4468741 IC50 = 3820.0 nM 5.42
CHEMBL4584081 IC50 = 6400.0 nM 5.19
CHEMBL4471895 IC50 = 6750.0 nM 5.17
CHEMBL4578338 IC50 = 10090.0 nM 5.00
CHEMBL3545369 EPACADOSTAT IC50 = 64500.0 nM 4.19
CHEMBL504816 (L)-1-METHYLTRYPTOPHAN IC50 - -