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Assay Detail

CHEMBL4356135

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Uncompetitive inhibition of recombinant human TDO expressed in Escherichia coli BL21 (DE3) assessed as assessed as inhibition constant using varying concentration of L-tryptophan as substrate by Dixon plot analysis
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tryptophan 2,3-dioxygenase (CHEMBL2140)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

N-Benzyl/Aryl Substituted Tryptanthrin as Dual Inhibitors of Indoleamine 2,3-Dioxygenase and Tryptophan 2,3-Dioxygenase.
Yang D, Zhang S, Fang X, Guo L, Hu N, Guo Z, Li X, Yang S, He JC, Kuang C, Yang Q.
J Med Chem (2019) 62 : 9161 -9174
PubMed 31580660 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 9 5.83 6.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4436480 1 6.92
CHEMBL4437046 1 6.51
CHEMBL4588805 1 6.47
CHEMBL4468741 1 5.91
CHEMBL4578338 1 5.81
CHEMBL4584081 1 5.63
CHEMBL4471895 1 4.75
CHEMBL3545369 EPACADOSTAT 3.0 1 4.66
CHEMBL504816 (L)-1-METHYLTRYPTOPHAN 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4436480 Ki = 120.0 nM 6.92
CHEMBL4437046 Ki = 310.0 nM 6.51
CHEMBL4588805 Ki = 340.0 nM 6.47
CHEMBL4468741 Ki = 1240.0 nM 5.91
CHEMBL4578338 Ki = 1560.0 nM 5.81
CHEMBL4584081 Ki = 2360.0 nM 5.63
CHEMBL4471895 Ki = 17960.0 nM 4.75
CHEMBL3545369 EPACADOSTAT Ki = 21700.0 nM 4.66
CHEMBL504816 (L)-1-METHYLTRYPTOPHAN Ki - -