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Assay Detail

CHEMBL4356374

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]NAMH from human H3R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysis
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H3 receptor (CHEMBL264)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

4-(3-Aminoazetidin-1-yl)pyrimidin-2-amines as High-Affinity Non-imidazole Histamine H3 Receptor Agonists with in Vivo Central Nervous System Activity.
Wágner G, Mocking TAM, Arimont M, Provensi G, Rani B, Silva-Marques B, Latacz G, Da Costa Pereira D, Karatzidou C, Vischer HF, Wijtmans M, Kieć-Kononowicz K, de Esch IJP, Leurs R.
J Med Chem (2019) 62 : 10848 -10866
PubMed 31675226 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 24 7.36 8.50

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4441731 1 8.50
CHEMBL4466700 1 8.20
CHEMBL4535316 1 8.00
CHEMBL4516714 1 7.90
CHEMBL90 HISTAMINE 4.0 1 7.90
CHEMBL4560428 1 7.80
CHEMBL4459469 1 7.80
CHEMBL4468740 1 7.50
CHEMBL4539015 1 7.40
CHEMBL4579823 1 7.40
CHEMBL4476584 1 7.40
CHEMBL4468827 1 7.30
CHEMBL4455352 1 7.30
CHEMBL4535363 1 7.30
CHEMBL4555457 1 7.20
CHEMBL4459723 1 7.10
CHEMBL4469111 1 7.10
CHEMBL4470781 1 7.00
CHEMBL4439165 1 7.00
CHEMBL4519616 1 6.90
CHEMBL4570258 1 6.90
CHEMBL4452204 1 6.70
CHEMBL4461302 1 6.60
CHEMBL4579385 1 6.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4441731 Ki = 3.162 nM 8.50
CHEMBL4466700 Ki = 6.31 nM 8.20
CHEMBL4535316 Ki = 10.0 nM 8.00
CHEMBL4516714 Ki = 12.59 nM 7.90
CHEMBL90 HISTAMINE Ki = 12.59 nM 7.90
CHEMBL4560428 Ki = 15.85 nM 7.80
CHEMBL4459469 Ki = 15.85 nM 7.80
CHEMBL4468740 Ki = 31.62 nM 7.50
CHEMBL4539015 Ki = 39.81 nM 7.40
CHEMBL4579823 Ki = 39.81 nM 7.40
CHEMBL4476584 Ki = 39.81 nM 7.40
CHEMBL4468827 Ki = 50.12 nM 7.30
CHEMBL4455352 Ki = 50.12 nM 7.30
CHEMBL4535363 Ki = 50.12 nM 7.30
CHEMBL4555457 Ki = 63.1 nM 7.20
CHEMBL4459723 Ki = 79.43 nM 7.10
CHEMBL4469111 Ki = 79.43 nM 7.10
CHEMBL4470781 Ki = 100.0 nM 7.00
CHEMBL4439165 Ki = 100.0 nM 7.00
CHEMBL4519616 Ki = 125.89 nM 6.90
CHEMBL4570258 Ki = 125.89 nM 6.90
CHEMBL4452204 Ki = 199.53 nM 6.70
CHEMBL4461302 Ki = 251.19 nM 6.60
CHEMBL4579385 Ki = 316.23 nM 6.50