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Assay Detail

CHEMBL4356375

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at human H3R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by CRE-luciferase reporter gene assay
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H3 receptor (CHEMBL264)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

4-(3-Aminoazetidin-1-yl)pyrimidin-2-amines as High-Affinity Non-imidazole Histamine H3 Receptor Agonists with in Vivo Central Nervous System Activity.
Wágner G, Mocking TAM, Arimont M, Provensi G, Rani B, Silva-Marques B, Latacz G, Da Costa Pereira D, Karatzidou C, Vischer HF, Wijtmans M, Kieć-Kononowicz K, de Esch IJP, Leurs R.
J Med Chem (2019) 62 : 10848 -10866
PubMed 31675226 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 24 8.06 9.50

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4441731 1 9.50
CHEMBL4535316 1 9.20
CHEMBL4560428 1 9.10
CHEMBL4466700 1 8.90
CHEMBL4516714 1 8.70
CHEMBL90 HISTAMINE 4.0 1 8.60
CHEMBL4476584 1 8.50
CHEMBL4455352 1 8.40
CHEMBL4579823 1 8.30
CHEMBL4459469 1 8.30
CHEMBL4539015 1 8.10
CHEMBL4555457 1 8.10
CHEMBL4468740 1 8.00
CHEMBL4469111 1 7.90
CHEMBL4470781 1 7.90
CHEMBL4468827 1 7.80
CHEMBL4535363 1 7.60
CHEMBL4570258 1 7.50
CHEMBL4459723 1 7.50
CHEMBL4579385 1 7.40
CHEMBL4461302 1 7.20
CHEMBL4452204 1 7.10
CHEMBL4519616 1 7.00
CHEMBL4439165 1 6.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4441731 EC50 = 0.3162 nM 9.50
CHEMBL4535316 EC50 = 0.631 nM 9.20
CHEMBL4560428 EC50 = 0.7943 nM 9.10
CHEMBL4466700 EC50 = 1.259 nM 8.90
CHEMBL4516714 EC50 = 1.995 nM 8.70
CHEMBL90 HISTAMINE EC50 = 2.512 nM 8.60
CHEMBL4476584 EC50 = 3.162 nM 8.50
CHEMBL4455352 EC50 = 3.981 nM 8.40
CHEMBL4579823 EC50 = 5.012 nM 8.30
CHEMBL4459469 EC50 = 5.012 nM 8.30
CHEMBL4539015 EC50 = 7.943 nM 8.10
CHEMBL4555457 EC50 = 7.943 nM 8.10
CHEMBL4468740 EC50 = 10.0 nM 8.00
CHEMBL4469111 EC50 = 12.59 nM 7.90
CHEMBL4470781 EC50 = 12.59 nM 7.90
CHEMBL4468827 EC50 = 15.85 nM 7.80
CHEMBL4535363 EC50 = 25.12 nM 7.60
CHEMBL4570258 EC50 = 31.62 nM 7.50
CHEMBL4459723 EC50 = 31.62 nM 7.50
CHEMBL4579385 EC50 = 39.81 nM 7.40
CHEMBL4461302 EC50 = 63.1 nM 7.20
CHEMBL4452204 EC50 = 79.43 nM 7.10
CHEMBL4519616 EC50 = 100.0 nM 7.00
CHEMBL4439165 EC50 = 158.49 nM 6.80