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Assay Detail

CHEMBL4356492

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant rat MGL expressed in human HeLa cells using 2-oleoylglycerol as substrate preincubated for 10 mins followed by substrate addition and measured after 10 mins by LC-MS analysis
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type HeLa
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Monoglyceride lipase (CHEMBL3321)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Benzisothiazolinone Derivatives as Potent Allosteric Monoacylglycerol Lipase Inhibitors That Functionally Mimic Sulfenylation of Regulatory Cysteines.
Castelli R, Scalvini L, Vacondio F, Lodola A, Anselmi M, Vezzosi S, Carmi C, Bassi M, Ferlenghi F, Rivara S, Møller IR, Rand KD, Daglian J, Wei D, Dotsey EY, Ahmed F, Jung KM, Stella N, Singh S, Mor M, Piomelli D.
J Med Chem (2020) 63 : 1261 -1280
PubMed 31714779 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 6.82 7.94

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4536072 1 7.94
CHEMBL4448448 1 7.78
CHEMBL4463191 1 7.71
CHEMBL4560077 1 7.66
CHEMBL2391916 1 7.47
CHEMBL4515209 1 7.27
CHEMBL3219170 1 7.26
CHEMBL1922513 1 7.23
CHEMBL1710047 1 7.22
CHEMBL4439900 1 7.03
CHEMBL4558017 1 6.87
CHEMBL4445264 1 6.79
CHEMBL4514686 1 6.72
CHEMBL4450432 1 6.71
CHEMBL4544505 1 6.62
CHEMBL4449771 1 6.56
CHEMBL297304 1 6.51
CHEMBL4553365 1 6.49
CHEMBL4530292 1 6.29
CHEMBL4438540 1 6.25
CHEMBL4576162 1 6.12
CHEMBL4548018 1 5.59
CHEMBL4545305 1 4.88

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4536072 IC50 = 11.5 nM 7.94
CHEMBL4448448 IC50 = 16.8 nM 7.78
CHEMBL4463191 IC50 = 19.5 nM 7.71
CHEMBL4560077 IC50 = 21.8 nM 7.66
CHEMBL2391916 IC50 = 34.1 nM 7.47
CHEMBL4515209 IC50 = 54.2 nM 7.27
CHEMBL3219170 IC50 = 55.5 nM 7.26
CHEMBL1922513 IC50 = 59.0 nM 7.23
CHEMBL1710047 IC50 = 59.5 nM 7.22
CHEMBL4439900 IC50 = 93.9 nM 7.03
CHEMBL4558017 IC50 = 135.0 nM 6.87
CHEMBL4445264 IC50 = 163.4 nM 6.79
CHEMBL4514686 IC50 = 189.4 nM 6.72
CHEMBL4450432 IC50 = 195.2 nM 6.71
CHEMBL4544505 IC50 = 237.9 nM 6.62
CHEMBL4449771 IC50 = 277.7 nM 6.56
CHEMBL297304 IC50 = 306.5 nM 6.51
CHEMBL4553365 IC50 = 322.1 nM 6.49
CHEMBL4530292 IC50 = 512.2 nM 6.29
CHEMBL4438540 IC50 = 568.0 nM 6.25
CHEMBL4576162 IC50 = 758.6 nM 6.12
CHEMBL4548018 IC50 = 2547.0 nM 5.59
CHEMBL4545305 IC50 = 13069.5 nM 4.88