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Assay Detail

CHEMBL4356496

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant rat MGL C201A/C208A mutant expressed in human HeLa cells using 2-oleoylglycerol as substrate preincubated for 10 mins followed by substrate addition and measured after 10 mins by LC-MS analysis
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type HeLa
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Monoglyceride lipase (CHEMBL3321)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Benzisothiazolinone Derivatives as Potent Allosteric Monoacylglycerol Lipase Inhibitors That Functionally Mimic Sulfenylation of Regulatory Cysteines.
Castelli R, Scalvini L, Vacondio F, Lodola A, Anselmi M, Vezzosi S, Carmi C, Bassi M, Ferlenghi F, Rivara S, Møller IR, Rand KD, Daglian J, Wei D, Dotsey EY, Ahmed F, Jung KM, Stella N, Singh S, Mor M, Piomelli D.
J Med Chem (2020) 63 : 1261 -1280
PubMed 31714779 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 5.96 6.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2391916 1 6.22
CHEMBL4463191 1 6.11
CHEMBL4536072 1 5.83
CHEMBL4448448 1 5.68

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2391916 IC50 = 604.7 nM 6.22
CHEMBL4463191 IC50 = 782.0 nM 6.11
CHEMBL4536072 IC50 = 1460.7 nM 5.83
CHEMBL4448448 IC50 = 2079.6 nM 5.68