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Assay Detail

CHEMBL4356683

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ERalpha in human MCF7:WS8 cells assessed as increase in degradation of ERalpha incubated for 24 hrs by In-Cell Western assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Estrogen receptor (CHEMBL206)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and Synthesis of Basic Selective Estrogen Receptor Degraders for Endocrine Therapy Resistant Breast Cancer.
Lu Y, Gutgesell LM, Xiong R, Zhao J, Li Y, Rosales CI, Hollas M, Shen Z, Gordon-Blake J, Dye K, Wang Y, Lee S, Chen H, He D, Dubrovyskyii O, Zhao H, Huang F, Lasek AW, Tonetti DA, Thatcher GRJ.
J Med Chem (2019) 62 : 11301 -11323
PubMed 31746603 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 8.65 9.59

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4527655 1 9.59
CHEMBL4586663 1 9.15
CHEMBL4532671 1 9.02
CHEMBL4515212 1 8.99
CHEMBL4533964 1 8.99
CHEMBL4524872 1 8.91
CHEMBL4543096 1 8.85
CHEMBL4483006 1 8.84
CHEMBL1358 FULVESTRANT 4.0 1 8.80
CHEMBL4516021 1 8.72
CHEMBL4448178 1 8.59
CHEMBL4436707 1 8.52
CHEMBL4578883 1 8.44
CHEMBL4564385 1 8.29
CHEMBL4440649 1 8.23
CHEMBL4436415 1 8.23
CHEMBL4464171 1 8.09
CHEMBL4538085 1 8.03
CHEMBL4447374 1 7.98

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4527655 IC50 = 0.257 nM 9.59
CHEMBL4586663 IC50 = 0.7079 nM 9.15
CHEMBL4532671 IC50 = 0.955 nM 9.02
CHEMBL4515212 IC50 = 1.023 nM 8.99
CHEMBL4533964 IC50 = 1.023 nM 8.99
CHEMBL4524872 IC50 = 1.23 nM 8.91
CHEMBL4543096 IC50 = 1.413 nM 8.85
CHEMBL4483006 IC50 = 1.445 nM 8.84
CHEMBL1358 FULVESTRANT IC50 = 1.585 nM 8.80
CHEMBL4516021 IC50 = 1.905 nM 8.72
CHEMBL4448178 IC50 = 2.57 nM 8.59
CHEMBL4436707 IC50 = 3.02 nM 8.52
CHEMBL4578883 IC50 = 3.631 nM 8.44
CHEMBL4564385 IC50 = 5.129 nM 8.29
CHEMBL4440649 IC50 = 5.888 nM 8.23
CHEMBL4436415 IC50 = 5.888 nM 8.23
CHEMBL4464171 IC50 = 8.128 nM 8.09
CHEMBL4538085 IC50 = 9.333 nM 8.03
CHEMBL4447374 IC50 = 10.47 nM 7.98