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Assay Detail

CHEMBL4359943

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of IRAK4 in human THP1-Xblue-MD2-CD14 cells assessed as reduction in LPS-induced NFkappaB transcription by measuring alkaline phosphatase level preincubated for 1 hr followed by LPS stimulation and measured after 20 hrs by quanti-blue reagent based reporter assay
40
Total Activities
40
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Interleukin-1 receptor-associated kinase 4 (CHEMBL3778)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of Potent and Selective Pyrazolopyrimidine IRAK4 Inhibitors.
Bryan MC, Drobnick J, Gobbi A, Kolesnikov A, Chen Y, Rajapaksa N, Ndubaku C, Feng J, Chang W, Francis R, Yu C, Choo EF, DeMent K, Ran Y, An L, Emson C, Huang Z, Sujatha-Bhaskar S, Brightbill H, DiPasquale A, Maher J, Wai J, McKenzie BS, Lupardus PJ, Zarrin AA, Kiefer JR.
J Med Chem (2019) 62 : 6223 -6240
PubMed 31082230 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 40 6.90 8.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4474636 1 8.10
CHEMBL4483551 1 7.60
CHEMBL4453861 1 7.46
CHEMBL4467170 1 7.42
CHEMBL3950656 1 7.39
CHEMBL4464832 1 7.28
CHEMBL4461516 1 7.28
CHEMBL4452936 1 7.25
CHEMBL4569547 1 7.25
CHEMBL4520118 1 7.25
CHEMBL4438027 1 7.22
CHEMBL4515682 1 7.22
CHEMBL4518559 1 7.18
CHEMBL4568867 1 7.12
CHEMBL4456286 1 7.08
CHEMBL4483477 1 7.01
CHEMBL4551768 1 7.01
CHEMBL4584820 1 7.01
CHEMBL4531013 1 6.89
CHEMBL4459509 1 6.85
CHEMBL4473269 1 6.77
CHEMBL4466517 1 6.77
CHEMBL4576141 1 6.77
CHEMBL4524886 1 6.64
CHEMBL4460055 1 6.60
CHEMBL4466092 1 6.48
CHEMBL4554579 1 6.48
CHEMBL4439253 1 6.46
CHEMBL3952937 1 6.43
CHEMBL4436128 1 6.04

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4474636 IC50 = 8.0 nM 8.10
CHEMBL4483551 IC50 = 25.0 nM 7.60
CHEMBL4453861 IC50 = 35.0 nM 7.46
CHEMBL4467170 IC50 = 38.0 nM 7.42
CHEMBL3950656 IC50 = 41.0 nM 7.39
CHEMBL4464832 IC50 = 53.0 nM 7.28
CHEMBL4461516 IC50 = 53.0 nM 7.28
CHEMBL4520118 IC50 = 56.0 nM 7.25
CHEMBL4452936 IC50 = 56.0 nM 7.25
CHEMBL4569547 IC50 = 56.0 nM 7.25
CHEMBL4515682 IC50 = 60.0 nM 7.22
CHEMBL4438027 IC50 = 60.0 nM 7.22
CHEMBL4518559 IC50 = 66.0 nM 7.18
CHEMBL4568867 IC50 = 76.0 nM 7.12
CHEMBL4456286 IC50 = 84.0 nM 7.08
CHEMBL4483477 IC50 = 97.0 nM 7.01
CHEMBL4584820 IC50 = 97.0 nM 7.01
CHEMBL4551768 IC50 = 98.0 nM 7.01
CHEMBL4531013 IC50 = 130.0 nM 6.89
CHEMBL4459509 IC50 = 140.0 nM 6.85
CHEMBL4473269 IC50 = 170.0 nM 6.77
CHEMBL4576141 IC50 = 170.0 nM 6.77
CHEMBL4466517 IC50 = 170.0 nM 6.77
CHEMBL4524886 IC50 = 230.0 nM 6.64
CHEMBL4460055 IC50 = 250.0 nM 6.60
CHEMBL4466092 IC50 = 330.0 nM 6.48
CHEMBL4554579 IC50 = 330.0 nM 6.48
CHEMBL4439253 IC50 = 350.0 nM 6.46
CHEMBL3952937 IC50 = 370.0 nM 6.43
CHEMBL4436128 IC50 = 910.0 nM 6.04
CHEMBL4462969 IC50 = 1200.0 nM 5.92
CHEMBL4460458 IC50 = 1300.0 nM 5.89
CHEMBL4544284 IC50 = 3000.0 nM 5.52
CHEMBL4550566 IC50 > 20000.0 nM -
CHEMBL4474664 IC50 > 20000.0 nM -
CHEMBL4463353 IC50 > 20000.0 nM -
CHEMBL3976860 IC50 - -
CHEMBL4569648 IC50 - -
CHEMBL4567291 IC50 - -
CHEMBL3979848 IC50 - -