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Assay Detail

CHEMBL4362852

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxicity in human NCI-H2228 cells harboring EML4-fused ALK variant 3 incubated for 72 hrs by alamar blue reagent based assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type NCI-H2228
Confidence 1 — Organism
Curated By Autocuration

Target

NCI-H2228 (CHEMBL1075536)
Type CELL-LINE
Organism Homo sapiens

Publication

Novel derivatives of anaplastic lymphoma kinase inhibitors: Synthesis, radiolabeling, and preliminary biological studies of fluoroethyl analogues of crizotinib, alectinib, and ceritinib.
Radaram B, Pisaneschi F, Rao Y, Yang P, Piwnica-Worms D, Alauddin MM.
Eur J Med Chem (2019) 182 : 111571 -111571
PubMed 31425908 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 6.17 8.19

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1738797 ALECTINIB 4.0 1 8.19
CHEMBL4474454 1 8.00
CHEMBL601719 CRIZOTINIB 4.0 1 5.55
CHEMBL2403108 CERITINIB 4.0 1 5.54
CHEMBL4566676 1 5.12
CHEMBL4532807 1 4.62

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1738797 ALECTINIB IC50 = 6.5 nM 8.19
CHEMBL4474454 IC50 = 10.0 nM 8.00
CHEMBL601719 CRIZOTINIB IC50 = 2800.0 nM 5.55
CHEMBL2403108 CERITINIB IC50 = 2900.0 nM 5.54
CHEMBL4566676 IC50 = 7500.0 nM 5.12
CHEMBL4532807 IC50 = 24200.0 nM 4.62