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Assay Detail

CHEMBL4369757

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of tyrosinase in mouse B16 cells using L-DOPA as substrate preincubated for 72 hrs followed by substrate addition and measured after 30 mins
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosinase (CHEMBL5346)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Thiosemicarbazones with tyrosinase inhibitory activity.
Hałdys K, Latajka R.
Medchemcomm (2019) 10 : 378 -389
PubMed 31015905 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 5.54 5.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3329471 1 5.92
CHEMBL74689 PHENYL(METHYL)THIOSEMICARBAZONE 1 5.70
CHEMBL243185 PHENYLTHIOSEMICARBAZONE 1 4.99
CHEMBL398206 1 -
CHEMBL440504 1 -
CHEMBL1779098 1 -
CHEMBL4540846 1 -
CHEMBL4435353 1 -
CHEMBL4593432 1 -
CHEMBL4440593 1 -
CHEMBL4472027 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3329471 IC50 = 1200.0 nM 5.92
CHEMBL74689 PHENYL(METHYL)THIOSEMICARBAZONE IC50 = 2000.0 nM 5.70
CHEMBL243185 PHENYLTHIOSEMICARBAZONE IC50 = 10200.0 nM 4.99
CHEMBL398206 IC50 = 139000.0 nM -
CHEMBL440504 IC50 < 1000.0 nM -
CHEMBL1779098 IC50 < 2300.0 nM -
CHEMBL4540846 IC50 < 1000.0 nM -
CHEMBL4435353 IC50 < 1000.0 nM -
CHEMBL4593432 IC50 < 1000.0 nM -
CHEMBL4440593 IC50 < 1000.0 nM -
CHEMBL4472027 IC50 < 1000.0 nM -