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Assay Detail

CHEMBL4370028

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Binding
Agonist activity at urotensin 2 receptor (unknown origin) expressed in HEK293 cells co-expressing Gq-polycistronic BRET biosensor assessed as induction of Gq activation measured for 5 mins by luminescence detection based BRET assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Urotensin-2 receptor (CHEMBL3764)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Functional Selectivity Revealed by N-Methylation Scanning of Human Urotensin II and Related Peptides.
Merlino F, Billard É, Yousif AM, Di Maro S, Brancaccio D, Abate L, Carotenuto A, Bellavita R, d'Emmanuele di Villa Bianca R, Santicioli P, Marinelli L, Novellino E, Hébert TE, Lubell WD, Chatenet D, Grieco P.
J Med Chem (2019) 62 : 1455 -1467
PubMed 30615452 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 12 8.16 8.73

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL218994 1 8.73
CHEMBL3104471 1 8.57
CHEMBL4474481 1 8.57
CHEMBL593662 UROTENSIN-II 1 8.52
CHEMBL4568153 1 8.44
CHEMBL4454498 1 8.33
CHEMBL4462940 1 8.28
CHEMBL4475783 1 8.25
CHEMBL4526244 1 7.86
CHEMBL4568539 1 7.73
CHEMBL4467241 1 7.65
CHEMBL4447811 1 6.98

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL218994 EC50 = 1.862 nM 8.73
CHEMBL3104471 EC50 = 2.692 nM 8.57
CHEMBL4474481 EC50 = 2.692 nM 8.57
CHEMBL593662 UROTENSIN-II EC50 = 3.02 nM 8.52
CHEMBL4568153 EC50 = 3.631 nM 8.44
CHEMBL4454498 EC50 = 4.677 nM 8.33
CHEMBL4462940 EC50 = 5.248 nM 8.28
CHEMBL4475783 EC50 = 5.623 nM 8.25
CHEMBL4526244 EC50 = 13.8 nM 7.86
CHEMBL4568539 EC50 = 18.62 nM 7.73
CHEMBL4467241 EC50 = 22.39 nM 7.65
CHEMBL4447811 EC50 = 104.71 nM 6.98