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Assay Detail

CHEMBL4370673

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant N-terminal GST-tagged CDK9 (2 to 372 residues) expressed in baculovirus infected Sf9 insect cells co-expressing human cyclin T1 (2 to 726 residues) after 40 mins in presence of [gamma-32P]ATP by scintillation counting analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

CDK9/cyclin T1 (CHEMBL2111389)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

A review on flavones targeting serine/threonine protein kinases for potential anticancer drugs.
Zhao L, Yuan X, Wang J, Feng Y, Ji F, Li Z, Bian J.
Bioorg Med Chem (2019) 27 : 677 -685
PubMed 30733087 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 6.72 6.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL16171 WOGONIN 1 6.72

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL16171 WOGONIN IC50 = 190.0 nM 6.72