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Assay Detail

CHEMBL4370675

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant CK2 expressed in Escherichia coli using RRRADDSDDDDD as substrate after 10 mins in presence of [gamma-32P]ATP
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Casein kinase 2 (CHEMBL3832943)
Type PROTEIN FAMILY
Organism Homo sapiens

Publication

A review on flavones targeting serine/threonine protein kinases for potential anticancer drugs.
Zhao L, Yuan X, Wang J, Feng Y, Ji F, Li Z, Bian J.
Bioorg Med Chem (2019) 27 : 677 -685
PubMed 30733087 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 5.85 6.46

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL31574 FISETIN 2.0 1 6.46
CHEMBL150 KAEMPFEROL 1.0 1 6.40
CHEMBL151 LUTEOLIN 2.0 1 6.30
CHEMBL50 QUERCETIN 3.0 1 6.26
CHEMBL28 APIGENIN 1 6.10
CHEMBL164 MYRICETIN 1 6.05
CHEMBL117 CHRYSIN 1 5.05
CHEMBL8260 BAICALEIN 2.0 1 5.00
CHEMBL28626 MORIN 1 5.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL31574 FISETIN IC50 = 350.0 nM 6.46
CHEMBL150 KAEMPFEROL IC50 = 400.0 nM 6.40
CHEMBL151 LUTEOLIN IC50 = 500.0 nM 6.30
CHEMBL50 QUERCETIN IC50 = 550.0 nM 6.26
CHEMBL28 APIGENIN IC50 = 800.0 nM 6.10
CHEMBL164 MYRICETIN IC50 = 900.0 nM 6.05
CHEMBL117 CHRYSIN IC50 = 9000.0 nM 5.05
CHEMBL8260 BAICALEIN IC50 = 10000.0 nM 5.00
CHEMBL28626 MORIN IC50 = 10000.0 nM 5.00