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Assay Detail

CHEMBL4371753

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NO711 binding to human GAT1 expressed in COS cell membranes preincubated for 25 mins by LC-ESI-MS/MS analysis
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium- and chloride-dependent GABA transporter 1 (CHEMBL1903)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel Allosteric Ligands of γ-Aminobutyric Acid Transporter 1 (GAT1) by MS Based Screening of Pseudostatic Hydrazone Libraries.
Hauke TJ, Wein T, Höfner G, Wanner KT.
J Med Chem (2018) 61 : 10310 -10332
PubMed 30376325 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 5.59 6.81

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1027 TIAGABINE 4.0 1 6.81
CHEMBL4525620 1 4.37
CHEMBL4560424 1 -
CHEMBL4563656 1 -
CHEMBL4537310 1 -
CHEMBL4436249 1 -
CHEMBL4526583 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1027 TIAGABINE IC50 = 154.88 nM 6.81
CHEMBL4525620 IC50 = 42657.95 nM 4.37
CHEMBL4560424 IC50 = 134896.29 nM -
CHEMBL4563656 IC50 = 134896.29 nM -
CHEMBL4537310 IC50 = 134896.29 nM -
CHEMBL4436249 IC50 = 134896.29 nM -
CHEMBL4526583 IC50 = 134896.29 nM -