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Assay Detail

CHEMBL4371902

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human GAT3 expressed in COS7 cells assessed as reduction in [2H6]GABA uptake preincubated for 25 mins followed by [2H6]GABA addition and measured after 6 mins by LC-MS/MS analysis
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium- and chloride-dependent GABA transporter 3 (CHEMBL5208)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Generation and screening of pseudostatic hydrazone libraries derived from 5-substituted nipecotic acid derivatives at the GABA transporter mGAT4.
Hauke TJ, Höfner G, Wanner KT.
Bioorg Med Chem (2019) 27 : 144 -152
PubMed 30503411 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 5.29 5.56

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4447559 1 5.56
CHEMBL75035 1 5.48
CHEMBL4566905 1 5.09
CHEMBL4548691 1 5.03

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4447559 IC50 = 2754.23 nM 5.56
CHEMBL75035 IC50 = 3311.31 nM 5.48
CHEMBL4566905 IC50 = 8128.31 nM 5.09
CHEMBL4548691 IC50 = 9332.54 nM 5.03