Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4375035

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of 20S proteasome beta 5i/20S proteasome beta 1i in human PBMC cell lysate assessed as reduction in chymotrypsin-like activity pre-incubated for 30 mins followed by Meo-Suc-GLF-AM substrate addition and measured after 30 mins by fluorescence assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Subcellular Lysate
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Design and Evaluation of Highly Selective Human Immunoproteasome Inhibitors Reveal a Compensatory Process That Preserves Immune Cell Viability.
Ladi E, Everett C, Stivala CE, Daniels BE, Durk MR, Harris SF, Huestis MP, Purkey HE, Staben ST, Augustin M, Blaesse M, Steinbacher S, Eidenschenk C, Pappu R, Siu M.
J Med Chem (2019) 62 : 7032 -7041
PubMed 31283222 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.15 8.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4516876 1 8.64
CHEMBL4542860 1 8.49
CHEMBL4543890 1 8.16
CHEMBL4542913 1 7.96
CHEMBL4558093 1 7.60
CHEMBL4580854 1 7.47
CHEMBL4445016 1 7.35
CHEMBL4437785 1 7.22
CHEMBL4452844 1 7.10
CHEMBL4458214 1 7.10
CHEMBL4460338 1 7.07
CHEMBL4519222 1 6.89
CHEMBL4468132 1 6.70
CHEMBL4465040 1 6.60
CHEMBL4470266 1 6.54
CHEMBL4435165 1 6.51
CHEMBL4565027 1 6.33
CHEMBL4573625 1 5.05

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4516876 IC50 = 2.3 nM 8.64
CHEMBL4542860 IC50 = 3.2 nM 8.49
CHEMBL4543890 IC50 = 6.9 nM 8.16
CHEMBL4542913 IC50 = 11.0 nM 7.96
CHEMBL4558093 IC50 = 25.0 nM 7.60
CHEMBL4580854 IC50 = 34.0 nM 7.47
CHEMBL4445016 IC50 = 45.0 nM 7.35
CHEMBL4437785 IC50 = 60.0 nM 7.22
CHEMBL4452844 IC50 = 80.0 nM 7.10
CHEMBL4458214 IC50 = 79.0 nM 7.10
CHEMBL4460338 IC50 = 85.0 nM 7.07
CHEMBL4519222 IC50 = 130.0 nM 6.89
CHEMBL4468132 IC50 = 200.0 nM 6.70
CHEMBL4465040 IC50 = 250.0 nM 6.60
CHEMBL4470266 IC50 = 290.0 nM 6.54
CHEMBL4435165 IC50 = 310.0 nM 6.51
CHEMBL4565027 IC50 = 470.0 nM 6.33
CHEMBL4573625 IC50 = 8900.0 nM 5.05