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Assay Detail

CHEMBL4375049

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human C-terminal His-tagged and N-terminal GST-tagged EGFR L858R/T790M/C797S triple mutant ( 668 to 1210 amino acids) expressed in baculovirus infected Sf9 insect cells using Poly(Glu,Tyr) 4:1 as substrate after 60 mins by ELISA
26
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-Based Design of 5-Methylpyrimidopyridone Derivatives as New Wild-Type Sparing Inhibitors of the Epidermal Growth Factor Receptor Triple Mutant (EGFR<sup>L858R/T790M/C797S</sup>).
Shen J, Zhang T, Zhu SJ, Sun M, Tong L, Lai M, Zhang R, Xu W, Wu R, Ding J, Yun CH, Xie H, Lu X, Ding K.
J Med Chem (2019) 62 : 7302 -7308
PubMed 31298540 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 7.13 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3545311 BRIGATINIB 4.0 1 8.52
CHEMBL4294743 1 8.24
CHEMBL4459978 2 7.56
CHEMBL4474196 2 7.56
CHEMBL4462965 2 7.43
CHEMBL4218431 1 7.41
CHEMBL4529308 2 7.25
CHEMBL4446258 2 7.19
CHEMBL4214567 1 7.14
CHEMBL4443062 1 7.14
CHEMBL4443413 2 7.09
CHEMBL4450934 2 7.05
CHEMBL4453276 2 6.64
CHEMBL4461479 2 -
CHEMBL4461253 1 -
CHEMBL4468949 1 -
CHEMBL4553136 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3545311 BRIGATINIB IC50 = 3.0 nM 8.52
CHEMBL4294743 IC50 = 5.8 nM 8.24
CHEMBL4474196 IC50 = 27.5 nM 7.56
CHEMBL4459978 IC50 = 27.7 nM 7.56
CHEMBL4462965 IC50 = 37.1 nM 7.43
CHEMBL4218431 IC50 = 38.7 nM 7.41
CHEMBL4529308 IC50 = 56.1 nM 7.25
CHEMBL4446258 IC50 = 65.1 nM 7.19
CHEMBL4443062 IC50 = 73.1 nM 7.14
CHEMBL4214567 IC50 = 73.1 nM 7.14
CHEMBL4443413 IC50 = 81.7 nM 7.09
CHEMBL4450934 IC50 = 88.6 nM 7.05
CHEMBL4462965 IC50 = 207.0 nM 6.68
CHEMBL4450934 IC50 = 224.1 nM 6.65
CHEMBL4453276 IC50 = 227.9 nM 6.64
CHEMBL4529308 IC50 = 498.1 nM 6.30
CHEMBL4459978 IC50 = 518.8 nM 6.29
CHEMBL4453276 IC50 = 587.6 nM 6.23
CHEMBL4461479 IC50 > 1000.0 nM -
CHEMBL4474196 IC50 > 1000.0 nM -
CHEMBL4443413 IC50 > 1000.0 nM -
CHEMBL4446258 IC50 > 1000.0 nM -
CHEMBL4461479 IC50 > 1000.0 nM -
CHEMBL4461253 IC50 > 1000.0 nM -
CHEMBL4468949 IC50 > 1000.0 nM -
CHEMBL4553136 IC50 > 1000.0 nM -