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Assay Detail

CHEMBL4375055

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Antagonist activity at beta1 adrenergic receptor (unknown origin) stably expressed in HEK293 cell membranes assessed as inhibition of isoproterenol-induced cAMP production preincubated for 15 mins followed by isoproterenol addition and measured after 30 mins by TR-FRET assay
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-1 adrenergic receptor (CHEMBL213)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Probing the Existence of a Metastable Binding Site at the β<sub>2</sub>-Adrenergic Receptor with Homobivalent Bitopic Ligands.
Gaiser BI, Danielsen M, Marcher-Rørsted E, Røpke Jørgensen K, Wróbel TM, Frykman M, Johansson H, Bräuner-Osborne H, Gloriam DE, Mathiesen JM, Sejer Pedersen D.
J Med Chem (2019) 62 : 7806 -7839
PubMed 31298548 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 7.00 8.33

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4514723 1 8.33
CHEMBL4465278 1 8.25
CHEMBL1160734 1 8.23
CHEMBL4434772 1 7.95
CHEMBL4441975 1 7.86
CHEMBL266195 ALPRENOLOL 2.0 1 7.86
CHEMBL4564189 1 7.84
CHEMBL4462252 1 7.71
CHEMBL4527821 1 7.70
CHEMBL4594173 1 7.58
CHEMBL4473518 1 7.56
CHEMBL4584173 1 7.55
CHEMBL4557995 1 7.01
CHEMBL4574652 1 6.90
CHEMBL4472564 1 6.74
CHEMBL41189 1 6.63
CHEMBL4446394 1 6.43
CHEMBL1395661 1 6.36
CHEMBL4442559 1 6.31
CHEMBL4459989 1 5.75
CHEMBL4450606 1 5.69
CHEMBL4530266 1 5.44
CHEMBL4556017 1 5.35
CHEMBL4474813 1 4.96

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4514723 IC50 = 4.677 nM 8.33
CHEMBL4465278 IC50 = 5.623 nM 8.25
CHEMBL1160734 IC50 = 5.888 nM 8.23
CHEMBL4434772 IC50 = 11.22 nM 7.95
CHEMBL4441975 IC50 = 13.8 nM 7.86
CHEMBL266195 ALPRENOLOL IC50 = 13.8 nM 7.86
CHEMBL4564189 IC50 = 14.45 nM 7.84
CHEMBL4462252 IC50 = 19.5 nM 7.71
CHEMBL4527821 IC50 = 19.95 nM 7.70
CHEMBL4594173 IC50 = 26.3 nM 7.58
CHEMBL4473518 IC50 = 27.54 nM 7.56
CHEMBL4584173 IC50 = 28.18 nM 7.55
CHEMBL4557995 IC50 = 97.72 nM 7.01
CHEMBL4574652 IC50 = 125.89 nM 6.90
CHEMBL4472564 IC50 = 181.97 nM 6.74
CHEMBL41189 IC50 = 234.42 nM 6.63
CHEMBL4446394 IC50 = 371.54 nM 6.43
CHEMBL1395661 IC50 = 436.52 nM 6.36
CHEMBL4442559 IC50 = 489.78 nM 6.31
CHEMBL4459989 IC50 = 1778.28 nM 5.75
CHEMBL4450606 IC50 = 2041.74 nM 5.69
CHEMBL4530266 IC50 = 3630.78 nM 5.44
CHEMBL4556017 IC50 = 4466.84 nM 5.35
CHEMBL4474813 IC50 = 10964.78 nM 4.96