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Assay Detail

CHEMBL4375058

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at beta2 adrenergic receptor (unknown origin) expressed in HEK293 cell assessed as inhibition of isoproterenol-induced cAMP production preincubated for 15 mins followed by isoproterenol addition and measured after 15 mins
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-2 adrenergic receptor (CHEMBL210)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Probing the Existence of a Metastable Binding Site at the β<sub>2</sub>-Adrenergic Receptor with Homobivalent Bitopic Ligands.
Gaiser BI, Danielsen M, Marcher-Rørsted E, Røpke Jørgensen K, Wróbel TM, Frykman M, Johansson H, Bräuner-Osborne H, Gloriam DE, Mathiesen JM, Sejer Pedersen D.
J Med Chem (2019) 62 : 7806 -7839
PubMed 31298548 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 8.40 9.14

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1160734 1 9.14
CHEMBL266195 ALPRENOLOL 2.0 1 8.94
CHEMBL4514723 1 8.92
CHEMBL4594173 1 8.81
CHEMBL4446394 1 8.80
CHEMBL4564189 1 8.75
CHEMBL4462252 1 8.69
CHEMBL4574652 1 8.66
CHEMBL1395661 1 8.63
CHEMBL4473518 1 8.49
CHEMBL4450606 1 8.48
CHEMBL4527821 1 8.28
CHEMBL4442559 1 8.20
CHEMBL4530266 1 7.92
CHEMBL4434772 1 7.86
CHEMBL4441975 1 7.59
CHEMBL4474813 1 6.67

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1160734 IC50 = 0.7244 nM 9.14
CHEMBL266195 ALPRENOLOL IC50 = 1.148 nM 8.94
CHEMBL4514723 IC50 = 1.202 nM 8.92
CHEMBL4594173 IC50 = 1.549 nM 8.81
CHEMBL4446394 IC50 = 1.585 nM 8.80
CHEMBL4564189 IC50 = 1.778 nM 8.75
CHEMBL4462252 IC50 = 2.042 nM 8.69
CHEMBL4574652 IC50 = 2.188 nM 8.66
CHEMBL1395661 IC50 = 2.344 nM 8.63
CHEMBL4473518 IC50 = 3.236 nM 8.49
CHEMBL4450606 IC50 = 3.311 nM 8.48
CHEMBL4527821 IC50 = 5.248 nM 8.28
CHEMBL4442559 IC50 = 6.31 nM 8.20
CHEMBL4530266 IC50 = 12.02 nM 7.92
CHEMBL4434772 IC50 = 13.8 nM 7.86
CHEMBL4441975 IC50 = 25.7 nM 7.59
CHEMBL4474813 IC50 = 213.8 nM 6.67