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Assay Detail

CHEMBL4375168

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal His6-tagged human PHGDH (4 to 315 residues) assessed as effect on NADH fluorescence incubated for 2 hrs using 3-PG substrate in presence of 250 uM NAD+ and PSAT1 by diaphorase based luminescence assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

D-3-phosphoglycerate dehydrogenase (CHEMBL2311243)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Intracellular Trapping of the Selective Phosphoglycerate Dehydrogenase (PHGDH) Inhibitor <b>BI-4924</b> Disrupts Serine Biosynthesis.
Weinstabl H, Treu M, Rinnenthal J, Zahn SK, Ettmayer P, Bader G, Dahmann G, Kessler D, Rumpel K, Mischerikow N, Savarese F, Gerstberger T, Mayer M, Zoephel A, Schnitzer R, Sommergruber W, Martinelli P, Arnhof H, Peric-Simov B, Hofbauer KS, Garavel G, Scherbantin Y, Mitzner S, Fett TN, Scholz G, Bruchhaus J, Burkard M, Kousek R, Ciftci T, Sharps B, Schrenk A, Harrer C, Haering D, Wolkerstorfer B, Zhang X, Lv X, Du A, Li D, Li Y, Quant J, Pearson M, McConnell DB.
J Med Chem (2019) 62 : 7976 -7997
PubMed 31365252 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 6.67 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4520837 1 8.52
CHEMBL4436264 1 8.40
CHEMBL4467246 1 8.10
CHEMBL4438014 1 7.02
CHEMBL4469992 1 6.64
CHEMBL4594097 1 6.61
CHEMBL4522467 1 6.60
CHEMBL4473052 1 6.52
CHEMBL4560125 1 6.47
CHEMBL4457646 1 6.46
CHEMBL4474620 1 6.38
CHEMBL4444192 1 6.15
CHEMBL4437057 1 6.13
CHEMBL4460522 1 5.89
CHEMBL4446511 1 5.63
CHEMBL4520181 1 5.17

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4520837 IC50 = 3.0 nM 8.52
CHEMBL4436264 IC50 = 4.0 nM 8.40
CHEMBL4467246 IC50 = 8.0 nM 8.10
CHEMBL4438014 IC50 = 95.0 nM 7.02
CHEMBL4469992 IC50 = 228.0 nM 6.64
CHEMBL4594097 IC50 = 246.0 nM 6.61
CHEMBL4522467 IC50 = 249.0 nM 6.60
CHEMBL4473052 IC50 = 301.0 nM 6.52
CHEMBL4560125 IC50 = 341.0 nM 6.47
CHEMBL4457646 IC50 = 345.0 nM 6.46
CHEMBL4474620 IC50 = 412.0 nM 6.38
CHEMBL4444192 IC50 = 714.0 nM 6.15
CHEMBL4437057 IC50 = 746.0 nM 6.13
CHEMBL4460522 IC50 = 1294.0 nM 5.89
CHEMBL4446511 IC50 = 2368.0 nM 5.63
CHEMBL4520181 IC50 = 6694.0 nM 5.17