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Assay Detail

CHEMBL4376060

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) assessed as thermal stabilization by sypro orange dye based thermal shift assay
20
Total Activities
19
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Kelch-like ECH-associated protein 1 (CHEMBL2069156)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

A Comparative Assessment Study of Known Small-Molecule Keap1-Nrf2 Protein-Protein Interaction Inhibitors: Chemical Synthesis, Binding Properties, and Cellular Activity.
Tran KT, Pallesen JS, Solbak SMØ, Narayanan D, Baig A, Zang J, Aguayo-Orozco A, Carmona RMC, Garcia AD, Bach A.
J Med Chem (2019) 62 : 8028 -8052
PubMed 31411465 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 19 5.02 5.72
Activity 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3819587 1 5.72
CHEMBL2402207 1 5.24
CHEMBL3237245 1 5.23
CHEMBL4077198 1 5.14
CHEMBL4440989 1 5.00
CHEMBL3632711 1 4.92
CHEMBL4534676 1 4.89
CHEMBL3899754 1 4.89
CHEMBL3423015 1 4.60
CHEMBL2381968 1 4.58
CHEMBL2402206 2 -
CHEMBL3617836 1 -
CHEMBL4167734 1 -
CHEMBL4576082 1 -
CHEMBL4164286 1 -
CHEMBL4166691 1 -
CHEMBL3134423 1 -
CHEMBL3235901 1 -
CHEMBL256097 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3819587 EC50 = 1900.0 nM 5.72
CHEMBL2402207 EC50 = 5800.0 nM 5.24
CHEMBL3237245 EC50 = 5900.0 nM 5.23
CHEMBL4077198 EC50 = 7200.0 nM 5.14
CHEMBL4440989 EC50 = 10000.0 nM 5.00
CHEMBL3632711 EC50 = 12000.0 nM 4.92
CHEMBL4534676 EC50 = 13000.0 nM 4.89
CHEMBL3899754 EC50 = 13000.0 nM 4.89
CHEMBL3423015 EC50 = 25000.0 nM 4.60
CHEMBL2381968 EC50 = 26000.0 nM 4.58
CHEMBL2402206 EC50 = 110000.0 nM -
CHEMBL3617836 EC50 <= 100000.0 nM -
CHEMBL4167734 EC50 <= 77000.0 nM -
CHEMBL4576082 EC50 <= 800000.0 nM -
CHEMBL4164286 EC50 <= 800000.0 nM -
CHEMBL4166691 EC50 <= 99000.0 nM -
CHEMBL3134423 EC50 <= 49000.0 nM -
CHEMBL3235901 EC50 <= 50000.0 nM -
CHEMBL256097 EC50 <= 25000.0 nM -
CHEMBL2402206 Activity - -