Assay Detail
Binding
CHEMBL4376072
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of FAM-LDEETGEFL-NH2 binding to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) measured after 30 to 60 mins in absence of TCEP buffer by fluorescence polarization assay
4
Total Activities
4
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Kelch-like ECH-associated protein 1 (CHEMBL2069156) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
A Comparative Assessment Study of Known Small-Molecule Keap1-Nrf2 Protein-Protein Interaction Inhibitors: Chemical Synthesis, Binding Properties, and Cellular Activity.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Inhibition | 3 | - | - |
| IC50 | 1 | 4.00 | 4.00 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3235901 | — | — | 1 | 4.00 |
| CHEMBL3617836 | — | — | 1 | - |
| CHEMBL3134423 | — | — | 1 | - |
| CHEMBL3235898 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3235901 | — | IC50 | = | 100000.0 | nM | 4.00 |
| CHEMBL3617836 | — | Inhibition | - | % | - | |
| CHEMBL3134423 | — | Inhibition | - | % | - | |
| CHEMBL3235898 | — | Inhibition | - | % | - |