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Assay Detail

CHEMBL4379571

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant N-terminal 6x-His-tagged c-KIT (547 to 935 residues)/(694 to 753 residues deletion) (unknown origin) expressed in baculovirus infected Sf9 insect cells using poly (Glu,Tyr) 4:1 as substrate measured after 150 mins in presence of ATP by ADP-glo reagent based luminescence assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mast/stem cell growth factor receptor Kit (CHEMBL1936)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of a Multitargeted Tyrosine Kinase Inhibitor for the Treatment of Gastrointestinal Stromal Tumors and Acute Myeloid Leukemia.
Lin WH, Wu SY, Yeh TK, Chen CT, Song JS, Shiao HY, Kuo CC, Hsu T, Lu CT, Wang PC, Wu TS, Peng YH, Lin HY, Chen CP, Weng YL, Kung FC, Wu MH, Su YC, Huang KW, Chou LH, Hsueh CC, Yen KJ, Kuo PC, Huang CL, Chen LT, Shih C, Tsai HJ, Jiaang WT.
J Med Chem (2019) 62 : 11135 -11150
PubMed 31721578 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 7.18 7.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4483070 1 7.62
CHEMBL4448198 1 7.54
CHEMBL4446778 1 7.33
CHEMBL535 SUNITINIB 4.0 1 7.32
CHEMBL4521178 1 7.31
CHEMBL4550794 1 7.31
CHEMBL4438245 1 7.28
CHEMBL941 IMATINIB 4.0 1 7.28
CHEMBL4513768 1 7.25
CHEMBL4457984 1 7.16
CHEMBL4546349 1 7.04
CHEMBL3593290 1 7.01
CHEMBL4452166 1 7.00
CHEMBL4475875 1 7.00
CHEMBL4589974 1 6.97
CHEMBL608533 MIDOSTAURIN 4.0 1 6.96
CHEMBL1946170 REGORAFENIB 4.0 1 6.94
CHEMBL4558996 1 6.91
CHEMBL4574485 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4483070 IC50 = 24.0 nM 7.62
CHEMBL4448198 IC50 = 29.0 nM 7.54
CHEMBL4446778 IC50 = 47.0 nM 7.33
CHEMBL535 SUNITINIB IC50 = 48.0 nM 7.32
CHEMBL4521178 IC50 = 49.0 nM 7.31
CHEMBL4550794 IC50 = 49.0 nM 7.31
CHEMBL4438245 IC50 = 53.0 nM 7.28
CHEMBL941 IMATINIB IC50 = 53.0 nM 7.28
CHEMBL4513768 IC50 = 56.0 nM 7.25
CHEMBL4457984 IC50 = 69.0 nM 7.16
CHEMBL4546349 IC50 = 91.0 nM 7.04
CHEMBL3593290 IC50 = 97.0 nM 7.01
CHEMBL4452166 IC50 = 100.0 nM 7.00
CHEMBL4475875 IC50 = 100.0 nM 7.00
CHEMBL4589974 IC50 = 107.0 nM 6.97
CHEMBL608533 MIDOSTAURIN IC50 = 109.0 nM 6.96
CHEMBL1946170 REGORAFENIB IC50 = 116.0 nM 6.94
CHEMBL4558996 IC50 = 123.0 nM 6.91
CHEMBL4574485 IC50 > 1000.0 nM -