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Assay Detail

CHEMBL4379983

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human DPP4 in pH 7.4 Tris buffer using AP-7-ATFMC as substrate preincubated for 15 mins followed by substrate addition by microplate reader analysis
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dipeptidyl peptidase 4 (CHEMBL284)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and discovery of triazolo-pyridazine-6-yl-substituted piperazines as effective anti-diabetic drugs; evaluated over dipeptidyl peptidase-4 inhibition mechanism and insulinotropic activities.
Bindu B, Vijayalakshmi S, Manikandan A.
Eur J Med Chem (2020) 187 : 111912 -111912
PubMed 31812034 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 8.83 9.12

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4454091 1 9.12
CHEMBL237500 LINAGLIPTIN 4.0 1 8.90
CHEMBL4460852 1 8.76
CHEMBL4547171 1 8.70
CHEMBL4529301 1 8.65

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4454091 IC50 = 0.75 nM 9.12
CHEMBL237500 LINAGLIPTIN IC50 = 1.25 nM 8.90
CHEMBL4460852 IC50 = 1.75 nM 8.76
CHEMBL4547171 IC50 = 2.0 nM 8.70
CHEMBL4529301 IC50 = 2.25 nM 8.65