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Assay Detail

CHEMBL4380518

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant full length human N-terminal His-tagged TBK1 using biotin-labelled Ahx-GDEDFSSFAEPG peptide as substrate preincubated with enzyme for 15 mins followed by substrate addition and further incubated for 30 mins in presence of 1 mM of ATP by TR-FRET assay
37
Total Activities
37
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase TBK1 (CHEMBL5408)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of BAY-985, a Highly Selective TBK1/IKKε Inhibitor.
Lefranc J, Schulze VK, Hillig RC, Briem H, Prinz F, Mengel A, Heinrich T, Balint J, Rengachari S, Irlbacher H, Stöckigt D, Bömer U, Bader B, Gradl SN, Nising CF, von Nussbaum F, Mumberg D, Panne D, Wengner AM.
J Med Chem (2020) 63 : 601 -612
PubMed 31859507 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 37 6.36 8.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4454902 1 8.30
CHEMBL4436188 1 8.22
CHEMBL4566796 1 8.15
CHEMBL4538751 1 7.77
CHEMBL4435393 1 7.75
CHEMBL4537673 BAY-985 1 7.52
CHEMBL577784 BX-795 1 7.35
CHEMBL4543618 1 7.30
CHEMBL4468020 1 6.86
CHEMBL4594167 1 6.81
CHEMBL4453292 1 6.78
CHEMBL4553949 1 6.43
CHEMBL4515413 1 6.41
CHEMBL4451595 1 6.41
CHEMBL4552254 1 6.36
CHEMBL4453481 1 6.28
CHEMBL4459120 1 6.21
CHEMBL4455930 1 6.08
CHEMBL4450510 1 5.90
CHEMBL4456062 1 5.86
CHEMBL4483062 1 5.72
CHEMBL4587843 1 5.69
CHEMBL4436416 1 5.68
CHEMBL4549459 1 5.64
CHEMBL4577511 1 5.60
CHEMBL4573638 1 5.58
CHEMBL4438389 1 5.43
CHEMBL4462056 1 5.29
CHEMBL4521346 1 5.29
CHEMBL4566710 1 5.28

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4454902 IC50 = 5.0 nM 8.30
CHEMBL4436188 IC50 = 6.0 nM 8.22
CHEMBL4566796 IC50 = 7.0 nM 8.15
CHEMBL4538751 IC50 = 17.0 nM 7.77
CHEMBL4435393 IC50 = 18.0 nM 7.75
CHEMBL4537673 BAY-985 IC50 = 30.0 nM 7.52
CHEMBL577784 BX-795 IC50 = 45.0 nM 7.35
CHEMBL4543618 IC50 = 50.0 nM 7.30
CHEMBL4468020 IC50 = 137.0 nM 6.86
CHEMBL4594167 IC50 = 155.0 nM 6.81
CHEMBL4453292 IC50 = 167.0 nM 6.78
CHEMBL4553949 IC50 = 372.0 nM 6.43
CHEMBL4515413 IC50 = 391.0 nM 6.41
CHEMBL4451595 IC50 = 388.0 nM 6.41
CHEMBL4552254 IC50 = 434.0 nM 6.36
CHEMBL4453481 IC50 = 530.0 nM 6.28
CHEMBL4459120 IC50 = 618.0 nM 6.21
CHEMBL4455930 IC50 = 829.0 nM 6.08
CHEMBL4450510 IC50 = 1270.0 nM 5.90
CHEMBL4456062 IC50 = 1390.0 nM 5.86
CHEMBL4483062 IC50 = 1920.0 nM 5.72
CHEMBL4587843 IC50 = 2040.0 nM 5.69
CHEMBL4436416 IC50 = 2100.0 nM 5.68
CHEMBL4549459 IC50 = 2270.0 nM 5.64
CHEMBL4577511 IC50 = 2490.0 nM 5.60
CHEMBL4573638 IC50 = 2660.0 nM 5.58
CHEMBL4438389 IC50 = 3740.0 nM 5.43
CHEMBL4521346 IC50 = 5110.0 nM 5.29
CHEMBL4462056 IC50 = 5070.0 nM 5.29
CHEMBL4566710 IC50 = 5290.0 nM 5.28
CHEMBL4575832 IC50 = 14700.0 nM 4.83
CHEMBL4461416 IC50 = 16700.0 nM 4.78
CHEMBL4538591 BAY-440 IC50 > 20000.0 nM -
CHEMBL4547610 IC50 > 20000.0 nM -
CHEMBL4466150 IC50 > 20000.0 nM -
CHEMBL4524534 IC50 > 20000.0 nM -
CHEMBL4545291 IC50 > 20000.0 nM -