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Assay Detail

CHEMBL4380993

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist at C3a receptor in human LAD2 cells assessed as inhibition of C3a-induced beta-hexosaminidase release preincubated for 30 mins followed by C3a addition
12
Total Activities
4
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type LAD2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

C3a anaphylatoxin chemotactic receptor (CHEMBL4761)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Potent Thiophene Antagonists of Human Complement C3a Receptor with Anti-Inflammatory Activity.
Rowley JA, Reid RC, Poon EKY, Wu KC, Lim J, Lohman RJ, Hamidon JK, Yau MK, Halili MA, Durek T, Iyer A, Fairlie DP.
J Med Chem (2020) 63 : 529 -541
PubMed 31910011 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 7.87 8.70
Inhibition 4 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4470864 3 8.70
CHEMBL4576800 3 8.40
CHEMBL4459627 3 8.10
CHEMBL389348 3 6.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4470864 IC50 = 2.0 nM 8.70
CHEMBL4470864 IC50 = 1.995 nM 8.70
CHEMBL4576800 IC50 = 4.0 nM 8.40
CHEMBL4576800 IC50 = 3.981 nM 8.40
CHEMBL4459627 IC50 = 8.0 nM 8.10
CHEMBL4459627 IC50 = 7.943 nM 8.10
CHEMBL389348 IC50 = 501.19 nM 6.30
CHEMBL389348 IC50 = 540.0 nM 6.27
CHEMBL389348 Inhibition - % -
CHEMBL4576800 Inhibition - % -
CHEMBL4459627 Inhibition - % -
CHEMBL4470864 Inhibition - % -