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Assay Detail

CHEMBL4382307

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of EGFR T790M/L858R double mutant (unknown origin) transfected in mouse BAF3 cells assessed as reduction in cell viability after 72 hrs by MTS assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type BaF3
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a potent dual ALK and EGFR T790M inhibitor.
Jang J, Son JB, To C, Bahcall M, Kim SY, Kang SY, Mushajiang M, Lee Y, Jänne PA, Choi HG, Gray NS.
Eur J Med Chem (2017) 136 : 497 -510
PubMed 28528303 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 16 7.33 8.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4448107 1 8.30
CHEMBL4473365 1 8.22
CHEMBL4565984 1 8.22
CHEMBL4449391 1 8.22
CHEMBL4467561 1 7.72
CHEMBL3353410 OSIMERTINIB 4.0 1 7.70
CHEMBL4531697 1 7.66
CHEMBL4540442 1 7.66
CHEMBL4581316 1 7.51
CHEMBL1229592 1 7.51
CHEMBL4520341 1 7.35
CHEMBL4558227 1 6.79
CHEMBL4516287 1 6.74
CHEMBL4465103 1 6.08
CHEMBL4533742 1 6.01
CHEMBL2403108 CERITINIB 4.0 1 5.60

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4448107 EC50 = 5.0 nM 8.30
CHEMBL4473365 EC50 = 6.0 nM 8.22
CHEMBL4565984 EC50 = 6.0 nM 8.22
CHEMBL4449391 EC50 = 6.0 nM 8.22
CHEMBL4467561 EC50 = 19.0 nM 7.72
CHEMBL3353410 OSIMERTINIB EC50 = 20.0 nM 7.70
CHEMBL4531697 EC50 = 22.0 nM 7.66
CHEMBL4540442 EC50 = 22.0 nM 7.66
CHEMBL4581316 EC50 = 31.0 nM 7.51
CHEMBL1229592 EC50 = 31.0 nM 7.51
CHEMBL4520341 EC50 = 45.0 nM 7.35
CHEMBL4558227 EC50 = 162.0 nM 6.79
CHEMBL4516287 EC50 = 183.0 nM 6.74
CHEMBL4465103 EC50 = 830.0 nM 6.08
CHEMBL4533742 EC50 = 972.0 nM 6.01
CHEMBL2403108 CERITINIB EC50 = 2500.0 nM 5.60