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Assay Detail

CHEMBL4383378

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]epibatidine from Lymnaea stagnalis acetylcholine-binding protein incubated for 60 mins followed by 3 hrs incubation in dark condition by microbeta counting analysis
30
Total Activities
29
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Lymnaea stagnalis
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acetylcholine-binding protein (CHEMBL6084)
Type SINGLE PROTEIN
Organism Lymnaea stagnalis

Publication

Discovery, cocrystallization and biological evaluation of novel piperidine derivatives as high affinity Ls-AChBP ligands possessing α7 nAChR activities.
Yang X, Shen J, Jiang L, Li W, Yu M, Pan G, Yan Y, Zhang C, Jia W, Xiao L, Yu H, Chen H, Zheng Y, Yu L, Xie Q, Zhou L, Shao L.
Eur J Med Chem (2018) 160 : 37 -48
PubMed 30317024 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 29 8.01 9.07
IC50 1 6.98 6.98

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4554047 1 9.07
CHEMBL4440256 1 8.64
CHEMBL4437999 1 8.47
CHEMBL4458658 1 8.46
CHEMBL4557873 1 8.28
CHEMBL4551353 1 8.28
CHEMBL4541936 1 8.25
CHEMBL4444365 1 8.23
CHEMBL4467323 1 8.22
CHEMBL4447538 1 8.01
CHEMBL4537917 1 7.94
CHEMBL4437423 1 7.93
CHEMBL4558905 1 7.92
CHEMBL4452235 1 7.91
CHEMBL4556242 1 7.90
CHEMBL4447431 1 7.89
CHEMBL4444981 1 7.80
CHEMBL4540765 1 7.78
CHEMBL4579917 1 7.78
CHEMBL4451952 1 7.76
CHEMBL4448437 1 7.72
CHEMBL4537587 1 7.65
CHEMBL4449862 1 7.58
CHEMBL4533711 1 7.54
CHEMBL4559828 2 7.15
CHEMBL4559380 1 -
CHEMBL4436100 1 -
CHEMBL4520291 1 -
CHEMBL4450187 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4554047 Ki = 0.86 nM 9.07
CHEMBL4440256 Ki = 2.27 nM 8.64
CHEMBL4437999 Ki = 3.39 nM 8.47
CHEMBL4458658 Ki = 3.47 nM 8.46
CHEMBL4551353 Ki = 5.22 nM 8.28
CHEMBL4557873 Ki = 5.3 nM 8.28
CHEMBL4541936 Ki = 5.57 nM 8.25
CHEMBL4444365 Ki = 5.93 nM 8.23
CHEMBL4467323 Ki = 6.02 nM 8.22
CHEMBL4447538 Ki = 9.77 nM 8.01
CHEMBL4537917 Ki = 11.41 nM 7.94
CHEMBL4437423 Ki = 11.72 nM 7.93
CHEMBL4558905 Ki = 11.92 nM 7.92
CHEMBL4452235 Ki = 12.42 nM 7.91
CHEMBL4556242 Ki = 12.48 nM 7.90
CHEMBL4447431 Ki = 13.0 nM 7.89
CHEMBL4444981 Ki = 15.96 nM 7.80
CHEMBL4540765 Ki = 16.47 nM 7.78
CHEMBL4579917 Ki = 16.5 nM 7.78
CHEMBL4451952 Ki = 17.22 nM 7.76
CHEMBL4448437 Ki = 18.84 nM 7.72
CHEMBL4537587 Ki = 22.23 nM 7.65
CHEMBL4449862 Ki = 26.19 nM 7.58
CHEMBL4533711 Ki = 29.02 nM 7.54
CHEMBL4559828 Ki = 71.5 nM 7.15
CHEMBL4559828 IC50 = 105.6 nM 6.98
CHEMBL4559380 Ki > 67.7 nM -
CHEMBL4436100 Ki > 67.7 nM -
CHEMBL4520291 Ki > 67.7 nM -
CHEMBL4450187 Ki > 67.7 nM -