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Assay Detail

CHEMBL4383937

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]N-alpha-methylhistamine from full length recombinant human H3R expressed in HEK293 cell membranes after 90 mins by beta scintillation counting method
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H3 receptor (CHEMBL264)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optimization and preclinical evaluation of novel histamine H<sub>3</sub>receptor ligands: Acetyl and propionyl phenoxyalkyl piperazine derivatives.
Szczepańska K, Karcz T, Kotańska M, Siwek A, Kuder KJ, Latacz G, Mogilski S, Hagenow S, Lubelska A, Sobolewski M, Stark H, Kieć-Kononowicz K.
Bioorg Med Chem (2018) 26 : 6056 -6066
PubMed 30448256 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 24 6.20 8.28

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4449790 1 8.28
CHEMBL4547780 1 7.99
CHEMBL4593213 1 7.81
CHEMBL4462632 1 6.94
CHEMBL4532780 1 6.72
CHEMBL4581913 1 6.50
CHEMBL4448015 1 6.42
CHEMBL4453714 1 6.30
CHEMBL4473431 1 6.04
CHEMBL4467220 1 5.91
CHEMBL4526298 1 5.86
CHEMBL4467342 1 5.86
CHEMBL4465046 1 5.84
CHEMBL4566960 1 5.79
CHEMBL4445569 1 5.76
CHEMBL4519189 1 5.63
CHEMBL4578432 1 5.61
CHEMBL4453108 1 5.47
CHEMBL4471691 1 5.44
CHEMBL4452580 1 5.43
CHEMBL4451316 1 5.39
CHEMBL4542051 1 5.31
CHEMBL4545685 1 -
CHEMBL4551195 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4449790 Ki = 5.2 nM 8.28
CHEMBL4547780 Ki = 10.2 nM 7.99
CHEMBL4593213 Ki = 15.4 nM 7.81
CHEMBL4462632 Ki = 115.0 nM 6.94
CHEMBL4532780 Ki = 192.0 nM 6.72
CHEMBL4581913 Ki = 315.0 nM 6.50
CHEMBL4448015 Ki = 377.0 nM 6.42
CHEMBL4453714 Ki = 499.0 nM 6.30
CHEMBL4473431 Ki = 908.0 nM 6.04
CHEMBL4467220 Ki = 1230.0 nM 5.91
CHEMBL4526298 Ki = 1392.0 nM 5.86
CHEMBL4467342 Ki = 1392.0 nM 5.86
CHEMBL4465046 Ki = 1444.0 nM 5.84
CHEMBL4566960 Ki = 1634.0 nM 5.79
CHEMBL4445569 Ki = 1720.0 nM 5.76
CHEMBL4519189 Ki = 2316.0 nM 5.63
CHEMBL4578432 Ki = 2453.0 nM 5.61
CHEMBL4453108 Ki = 3390.0 nM 5.47
CHEMBL4471691 Ki = 3627.0 nM 5.44
CHEMBL4452580 Ki = 3697.0 nM 5.43
CHEMBL4451316 Ki = 4040.0 nM 5.39
CHEMBL4542051 Ki = 4889.0 nM 5.31
CHEMBL4545685 Ki > 10000.0 nM -
CHEMBL4551195 Ki > 10000.0 nM -