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Assay Detail

CHEMBL4384432

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human wild type GST-tagged EGFR (668 to 1210 residues) expressed in baculovirus expression system after 1 hr by kinase tracer 199 based TR-FRET assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Balancing reactivity and antitumor activity: heteroarylthioacetamide derivatives as potent and time-dependent inhibitors of EGFR.
Castelli R, Bozza N, Cavazzoni A, Bonelli M, Vacondio F, Ferlenghi F, Callegari D, Silva C, Rivara S, Lodola A, Digiacomo G, Fumarola C, Alfieri R, Petronini PG, Mor M.
Eur J Med Chem (2019) 162 : 507 -524
PubMed 30472599 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 9.27 9.47

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4449681 1 9.47
CHEMBL939 GEFITINIB 4.0 1 9.33
CHEMBL4530170 1 9.28
CHEMBL4439974 1 9.28
CHEMBL4526992 1 9.21
CHEMBL4440618 1 9.06
CHEMBL4572315 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4449681 IC50 = 0.34 nM 9.47
CHEMBL939 GEFITINIB IC50 = 0.47 nM 9.33
CHEMBL4530170 IC50 = 0.53 nM 9.28
CHEMBL4439974 IC50 = 0.52 nM 9.28
CHEMBL4526992 IC50 = 0.62 nM 9.21
CHEMBL4440618 IC50 = 0.87 nM 9.06
CHEMBL4572315 IC50 - -